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Sally Sue and Peggy Sue User Guide

Chapter 1:

Let’s Get Started

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1

Welcome . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2

Simple Western Assays. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2

Chapter 2:

Getting Your Lab Ready

. . . . . . . . . . . . . . . . . . . . 3

Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4

Space Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4

Physical Specifications. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5

Electrical Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6

Environmental Requirements. . . . . . . . . . . . . . . . . . . . . . . . . 6

Software and Computer Requirements . . . . . . . . . . . . . . . 7

General Guidelines and Information . . . . . . . . . . . . . . . . . . 7

Intended Use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

Lifting and Moving the System: Lift Sally Sue and
Peggy Sue Correctly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

Chapter 3:

Sally Sue and Peggy Sue

. . . . . . . . . . . . . . . . . . . . 9

Instrument Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10

External Components . . . . . . . . . . . . . . . . . . . . . . . . . . . 10

Internal Components . . . . . . . . . . . . . . . . . . . . . . . . . . . 14

Auxiliary Module. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28

Removing and Installing Bottles . . . . . . . . . . . . . . . . 29

Connection Panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30

Chapter 4:

Operating Sally Sue and Peggy Sue

. . . . . . 33

Before You Throw the Switch . . . . . . . . . . . . . . . . . . . . . . . .34

Power Up . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .34

Starting a Run: Size-based Assays (Sally Sue and Peggy
Sue) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .34

Step 1 - Get Ready . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .34

Step 2 - Start the Run . . . . . . . . . . . . . . . . . . . . . . . . . . . .35

Step 3 - Post-Run Procedures . . . . . . . . . . . . . . . . . . . .42

Starting a Run: Charge-based Assays (Peggy Sue

Only). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .43

Step 1 - Get Ready . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .43

Step 2 - Start the Run . . . . . . . . . . . . . . . . . . . . . . . . . . . .43

Step 3 - Post-Run Procedures . . . . . . . . . . . . . . . . . . . .50

Stopping a Run . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .51

Waste and Water Bottles. . . . . . . . . . . . . . . . . . . . . . . . . . . . .51

Controlling Sally Sue and Peggy Sue . . . . . . . . . . . . . . . . .52

Starting a New Run . . . . . . . . . . . . . . . . . . . . . . . . . . . . .52

Opening Trays . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .53

Cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .53

Cleaning After a Run Error. . . . . . . . . . . . . . . . . . . . . . .55

Self Test . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .56

Viewing and Changing System Properties. . . . . . .57

Viewing Error and Test Logs . . . . . . . . . . . . . . . . . . . . .58

Instrument Status Modes . . . . . . . . . . . . . . . . . . . . . . . . . . . .59

Table of Contents

Summary of Contents for ProteinSimple Peggy Sue

Page 1: ... Sue User Guide Copyright 2017 ProteinSimple All rights reserved ProteinSimple 3001 Orchard Parkway San Jose CA 95134 Toll free 888 607 9692 Tel 408 510 5500 Fax 408 510 5599 email support proteinsimple com web proteinsimple com ...

Page 2: ...e s Simple Western technology is covered by issued and pending patents in the U S and other countries For more information please see http www proteinsimple com Intellectual_Property html ProteinSimple and the ProteinSimple logo are trademarks or registered trademarks of ProteinSimple Other marks appearing in these materials are marks of their respective owners ...

Page 3: ...4 Auxiliary Module 28 Removing and Installing Bottles 29 Connection Panel 30 Chapter 4 Operating Sally Sue and Peggy Sue 33 Before You Throw the Switch 34 Power Up 34 Starting a Run Size based Assays Sally Sue and Peggy Sue 34 Step 1 Get Ready 34 Step 2 Start the Run 35 Step 3 Post Run Procedures 42 Starting a Run Charge based Assays Peggy Sue Only 43 Step 1 Get Ready 43 Step 2 Start the Run 43 St...

Page 4: ...nt 70 Storage 70 Shipment Preparation 71 Spare Parts 71 Troubleshooting 71 Chapter 6 General Information 73 Compliance 74 Safety Guidelines 74 Door Interlock 75 System Trays 75 Auxiliary Module Reservoirs 75 Chemical Hazards 76 Chemical Waste Hazards 77 Waste Production and Disposal 78 Safety Data Sheets 79 Instrument Safety Labels 79 Customer Service and Technical Support 80 Legal Notices 81 Sall...

Page 5: ...page 1 Sally Sue and Peggy Sue User Guide Chapter 1 Let s Get Started Chapter Overview Welcome Simple Western Assays ...

Page 6: ...you or damage to Sally Sue and Peggy Sue WARNING Warns you that serious physical injury can result if the listed precautions aren t followed Simple Western Assays A Simple Western assay is an automated Western no gels no transfer devices no blots no film and no man ual analysis Simple Westerns take place in a capillary Sally Sue and Peggy Sue automate all steps of the process for you including sam...

Page 7: ...er Guide Chapter 2 Getting Your Lab Ready Chapter Overview Introduction Space Requirements Physical Specifications Electrical Requirements Environmental Requirements Software and Computer Requirements General Guidelines and Information ...

Page 8: ...ace Requirements You ll need a lab bench or table that can support 200 lbs 91 kg and that has enough space for both the instrument and computer There should be sufficient clearance for both heat ventilation and to provide access if Sally Sue or Peggy Sue ever need service IMPORTANT Sally Sue and Peggy Sue need a stable surface and must remain level to work properly The lab bench or table can t shi...

Page 9: ...00 feet Table 2 2 Physical specifications Dimension Meters Feet Peggy Sue Width 0 94 3 08 Width with doors open 1 27 4 17 Depth 0 61 2 00 Height 0 84 2 76 Auxiliary Module Width 0 33 1 08 Depth 0 30 1 00 Height 0 51 1 67 Tubing between Auxiliary Mod ule and instrument 3 00 9 84 Computer Workstation Width 0 66 2 17 Depth 0 76 2 49 Height 0 41 1 35 ...

Page 10: ...uirements we recommend the grounded circuits terminate at the receptacles and receptacles must be located within 10 ft 3 m of the instrument Environmental Requirements Sally Sue and Peggy Sue like a consistent temperature in the lab They work best when conditions stay within these ranges Table 2 4 Environmental requirements Region Volts AC Frequency Hz Amps US and Canada 120 60 5 5 Europe 240 50 2...

Page 11: ...parate workstation such as your desktop computer Your computer must meet the minimum requirements listed in the table below to run the software and process data Table 2 5 Computer requirements General Guidelines and Information Intended Use NOTE Sally Sue and Peggy Sue are for research use only Not for use in diagnostic procedures Lifting and Moving the System Lift Sally Sue and Peggy Sue Correctl...

Page 12: ...page 8 Chapter 2 Getting Your Lab Ready Sally Sue and Peggy Sue User Guide ...

Page 13: ...page 9 Sally Sue and Peggy User Guide Chapter 3 Sally Sue and Peggy Sue Chapter Overview Instrument Overview Auxiliary Module ...

Page 14: ...e and Peggy Sue Sally Sue and Peggy Sue User Guide Instrument Overview Sally Sue s and Peggy Sue s individual hardware components are described in this section External Components System Door System Door Status Lights Left Right ...

Page 15: ...is on Using the handle to the right of the System Status Indicators pull the right door open first Open the left door by pulling forward on the top and bottom latches To close the doors close the left door first and make sure both latches engage then close the right door The right door uses a magnet so it closes properly NOTE Sally Sue s and Peggy Sue s doors must be closed before starting a run o...

Page 16: ... the system status is ready green and the doors are open the robot s system tray Z stage motors and UV power supply are disabled WARNING Don t override the door interlock The interlock protects you from exposure to UV light and a poten tial pinch hazard that could be caused by moving system trays Status Lights The LEDs on Sally Sue s and Peggy Sue s front panel tell you what their doing Ready gree...

Page 17: ...to make sure Sally Sue and Peggy Sue are level and their level will also be checked during preventive maintenance visits The level status of Sally Sue and Peggy Sue are monitored internally and reported to you through Compass for Simple Western NOTE If Sally Sue or Peggy Sue need to be moved from their original install location or require leveling please see Leveling the Instrument on page 64 for ...

Page 18: ...ich is used to pick up and transfer capillaries Gripper The gripper sits at the base of the head assembly and is used to transfer capillaries between system trays The gripper picks up 12 capillaries at a time from the capillary box and moves them to and from each tray during each cycle in a run The gripper rotates 90 degrees to allow vertical transfer and horizontal placement of capillaries Head A...

Page 19: ... the sample resource incubator separation and capillary discard trays during the run It also moves the pipettor that s used to transfer fluids and perform washes inside the instrument Syringe Pump The syringe pump pipettes DI water reagents in the resource tray cups and also assists in washing the man ifold head per the assay protocol ...

Page 20: ...onizer Fans Anti static ionizer fans are located inside Sally Sue s or Peggy Sue s right door They re used to circulate ion ized air inside the system The uniform static charge they provide makes sure capillaries are always trans ferred properly between system trays during a run ...

Page 21: ...y and reagent storage and to carry out different phases of the assay You can open and close any tray by selecting Open Trays from the Instrument menu in Compass for Simple Western For more details please refer to the Opening Trays on page 53 Separation Tray Incubator Tray Upper Incubator Tray Lower Resource Tray Sample Tray Capillary Discard Tray ...

Page 22: ...t Running Buffer middle Matrix Removal Buffer right If you ll only be using one box of capillaries put it in the primary box location closest to the reagents NOTE You can also use the labels on the resource tray as a guide to where to insert reagents and capillar ies Charge based Assays Peggy Sue only For charge assays the resource tray holds Wash Buffer Anolyte Catholyte and up to two capillary b...

Page 23: ...r left Anolyte middle Catholyte right If you ll only be using one box of capillaries put it in the primary box location closest to the reagents NOTE You can also use the labels on the resource tray as a guide for where to insert reagents and capillar ies Capillary Boxes Wash Buffer Catholyte Anolyte ...

Page 24: ...your samples and reagents It uses a cold block to keep a portion of the plate at 10 C The remaining portion of the plate stays at ambient instrument temperature All sample plates must have lids and should be inserted into the sample tray so the A1 well position is at the upper left corner of the cold block Cold Block A1 Position Sample Plate ...

Page 25: ...r Simple Western will then open the sample tray to allow you to put a lid on the plate When you insert the sample plate make sure it s firmly seated and level on the cold block When plates aren t level they can interfere with the movement of the sample tray Incubation Trays Sally Sue and Peggy Sue use two incubator trays to perform the incubation steps in an assay protocol Each tray holds up to 48...

Page 26: ...ed with buffer s and Sally Sue and Peggy Sue will also remove the buffer s and wash the troughs once the separation step is complete Chemilumi nescent and fluorescent CCD images are taken of the separation in the capillaries through the detection window Size based assays both front and rear troughs are used for Running Buffer Charge based assays the front trough is used for Anolyte and the rear fo...

Page 27: ...rea to catch capillaries and emptying the lid on a daily basis WARNING SHARPS HAZARD Capillaries may present a potential sharps hazard Dispose of used capillaries according to your insti tution s sharps disposal policy NOTE An overfilled capillary discard tray can cause the sample tray to jam If this happens call Protein Simple Technical Support at 888 607 9692 or 408 510 5500 option 3 Plate Lid D...

Page 28: ...fold Wash Station The manifold wash station washes and cleans the instrument manifold after each separation and prevents build up of viscous materials in the head NOTE Replace the manifold sponge with a new one after each run and whenever you start an experiment on a new assay plate ...

Page 29: ...nel of the instrument that can be used for instrument clean ing procedures or for cleaning liquid spills The vacuum turns on whenever Sally Sue s or Peggy Sue s doors and system tray are open at the same time To use the vacuum turn the valve to the on position Turn the valve back to the off position when you aren t using it ...

Page 30: ...supply cord provided with Sally Sue or Peggy Sue If the cord is damaged please contact ProteinSimple Technical Support WARNING No user replaceable parts WARNING SHOCK HAZARD Disconnect the power cord from the instrument power input to disconnect power to the instru ment Network Connection A 10 100 BASE T Ethernet RJ 45 connector is used to connect Sally Sue and Peggy Sue to a computer or local net...

Page 31: ...in the auxiliary module This line is under vacuum when waste aspirations are performed Vacuum Vacuum is provided to the instrument by the auxiliary module and is used in pick and place operations inside the instrument Water Provides water to the instrument from the auxiliary module Instrument Rear Panel System Label A system label can be found on Sally Sue s and Peggy Sue s rear panel It includes ...

Page 32: ...roduction please refer to the Waste Production and Disposal on page 78 There are two liquid level sensors in each bottle The sensor at the bottom of the bottle detects when the bottle is empty and the sensor at the top of the bottle determines if sufficient volume is available to com plete an eight cycle assay with the instrument NOTE We recommend you check fluid levels in the bottles before start...

Page 33: ...nstalling Bottles Bottles may be removed from the auxiliary module whenever Sally Sue or Peggy Sue are idle To remove or install the waste or water bottle use the following procedure IMPORTANT Don t remove either bottle if Sally Sue or Peggy Sue are running doing this may abort the run in progress 1 Remove the bottle by unscrewing it clockwise until it disengages from the manifold 2 Pull the bottl...

Page 34: ...ons bmbl5 Depending on the samples used waste bottle contents may constitute a biohazard Use precaution when emptying the waste bottle Dispose of waste bottle contents in accor dance with good laboratory practices and local state provincial or national environmen tal and health regulations Read and understand the Safety Data Sheets SDSs provided by the manufacturers of the chemicals in the waste v...

Page 35: ...Module page 31 Sally Sue and Peggy Sue User Guide Vacuum Vacuum is provided to the instrument and is used in pick and place operations inside the instrument Water This connection provides water to the instrument ...

Page 36: ...page 32 Chapter 3 Sally Sue and Peggy Sue Sally Sue and Peggy Sue User Guide ...

Page 37: ...Peggy Sue Chapter Overview Before You Throw the Switch Power Up Starting a Run Size based Assays Sally Sue and Peggy Sue Starting a Run Charge based Assays Peggy Sue Only Stopping a Run Waste and Water Bottles Controlling Sally Sue and Peggy Sue Instrument Status Modes Shutdown ...

Page 38: ...for the instrument to initialize 4 Double click the Compass for Simple Western icon to open the application Starting a Run Size based Assays Sally Sue and Peggy Sue Step 1 Get Ready 1 Create or open your assay file in Compass for Simple Western 2 Prepare the instrument empty waste refill water and add a new manifold sponge 3 Prepare the assay plate using the procedure in the product insert IMPORTA...

Page 39: ...e main menu and click Open Assay b A list of the last five assays opened will display Select one of these assays or click Browse to open the Assay folder and select a different one 2 To start a run with a new assay a Select File in the main menu click Open Assay and select Sally Sue Size or Peggy Sue Size From the pop up window select the Assay Type Size Total Protein Size or Charge Peggy Sue only...

Page 40: ...d NOTE If you didn t clean the manifold in Sally Sue or Peggy Sue prior to starting the run a message will appear If you see this message click Yes to cancel the run and perform the manifold cleaning 4 Check Water and Waste The fluid levels in the Auxiliary Module bottles will be checked by the soft ware If the levels in both bottles will allow Sally Sue or Peggy Sue to complete the run the wizard...

Page 41: ...eed 5 Replace Sponge Each time you start a new run you should use a new sponge Remove and discard the old sponge and insert a new one in the manifold wash station 6 Start The resource tray will open a If you re running an assay with Running Buffer Rev B fill the cups with Wash Buffer Running Buf fer and Matrix Removal Buffer If you re running a split Running Buffer assay fill the cups with Wash Bu...

Page 42: ...ional capillaries are needed use the secondary position When the primary box is empty Sally Sue or Peggy Sue will automatically move to the secondary box Discard any leftover Running Buffer Rev B Upper or Lower Wash Buffer and Matrix Removal Buffer in the resource tray cups before refilling them for new run NOTE You can also use the labels on the resource tray as a guide for where to insert reagen...

Page 43: ...based Assays Sally Sue and Peggy Sue page 39 Sally Sue and Peggy Sue User Guide Primary Capillary Box Secondary Capillary Box Wash Buffer Matrix Removal Buffer or Running Buffer or Upper Running Buffer Lower Running Buffer ...

Page 44: ... Sue User Guide 7 Load Sample Plate The sample tray will open Place the lidded 384 well plate on the cooling block in the tray making sure the A1 well position is in the upper left corner of the cold block Once this is done click Next The sample tray will close A1 Position ...

Page 45: ...you insert the sample plate make sure it s firmly seated and level on the cold block When plates aren t level they can interfere with the movement of the sample tray 8 Begin the Automated Run The data file name will automatically default to the assay name appended with the current date and time To change the file name begin typing in the text box To change the directory where the data file will be...

Page 46: ...o your insti tution s sharps disposal policy WARNING BIOHAZARD Samples and waste bottle contents should be handled by procedures recommended in the CDC NIH manual Biosafety in Microbiological and Biomedical Laboratories BMBL The manual is available from the U S Government Printing Office or online at http www cdc gov biosafety publications bmbl5 Depending on the samples used waste bottle contents ...

Page 47: ...ration and get the best results keep a lid on the assay plate until ready to use 4 While the plate is spinning add Wash Buffer Anolyte and Catholyte to resource tray cups and insert a capillary box in the resource tray IMPORTANT Capillaries are light sensitive Keep the cover on the box until you re ready to transfer the capillary box to the resource tray 5 Place the assay plate into the sample tra...

Page 48: ... Assay and select Peggy Sue Charge to get the default Peggy Sue assay conditions b The Start button will display which means an assay is already loaded a Go to the Assay screen and verify this is the assay you want to use If not select File in the main menu click Open Assay and select another one 3 Click Start This will launch the Start Run Wizard NOTE If you didn t clean the manifold in Peggy Sue...

Page 49: ...Sue to complete the run the wizard screen will display Water Level OK and Waste Level OK messages Click Next to proceed NOTE If the waste level is too high or the water level is too low to complete the run a message will display in this screen If this happens fill or empty each bottle using the procedures in Removing and Installing Bottles on page 29 Once this is done error status will automatical...

Page 50: ...the primary position Add a secondary capillary box if more capillaries are required for you run Click Next when you re done The resource tray will close NOTES Make sure enough capillaries are loaded to complete the run Insert a capillary box in the primary position first and if additional capillaries are needed use the secondary position When the primary box is empty Peggy Sue will automatically m...

Page 51: ...y Sue Only page 47 Sally Sue and Peggy Sue User Guide NOTE You can also use the labels on the resource tray as a guide for where to insert reagents and capillar ies Primary Capillary Box Secondary Capillary Box Wash Buffer Catholyte Anolyte ...

Page 52: ... Sue User Guide 7 Load Sample Plate The sample tray will open Place the lidded 384 well plate on the cooling block in the tray making sure the A1 well position is in the upper left corner of the cold block Once this is done click Next The sample tray will close A1 Position ...

Page 53: ... the sample plate make sure it s firmly seated and level on the cold block When plates aren t level they can interfere with the movement of the sample tray 8 Begin the Automated Run The data file name will automatically default to the assay name appended with the current date and time To change the file name begin typing in the text box To change the directory where the data file will be stored cl...

Page 54: ...your insti tution s sharps disposal policy WARNING BIOHAZARD Samples and waste bottle contents should be handled by procedures recommended in the CDC NIH manual Biosafety in Microbiological and Biomedical Laboratories BMBL The manual is available from the U S Government Printing Office or online at http www cdc gov biosafety publications bmbl5 Depending on the samples used waste bottle contents ma...

Page 55: ...tles You should empty the waste bottle and refill the water bottle in the Auxiliary Module before starting a new run To remove or install bottles IMPORTANT Do not remove either bottle if Sally Sue or Peggy Sue are running doing this may abort the run in progress 1 Remove the bottle by unscrewing it clockwise until it disengages from the manifold 2 Pull the bottle down slowly until the sensor at th...

Page 56: ...ard Use precaution when emptying the waste bottle Dispose of waste bottle contents in accor dance with good laboratory practices and local state provincial or national environmen tal and health regulations Controlling Sally Sue and Peggy Sue The Instrument menu allows you to control Sally Sue and Peggy Sue NOTE Instrument menu options are only active when a computer with Compass for Simple Western...

Page 57: ...ay by clicking its button The button will change color when a tray is open NOTE Only one tray can be open at a time To close a tray click its tray button again NOTE If the tray control window is closed when a tray is open the tray will close automatically Cleaning Instrument cleaning can be performed either manually or automatically in Compass for Simple Western Resource Tray Sample Tray Resource ...

Page 58: ...mpty reservoir cups To run the pipet wash you ll need Compass for Simple Western v2 6 7 and up on page 65 for the manual manifold cleaning procedure NOTE Please contact Protein Simple Technical Support if you have any questions on the manifold clean ing procedure Cleanup This option is a fully automated cleaning step The manifold head is flushed the separation tray troughs are aspirated and washed...

Page 59: ...en cleaning is done instrument status will change to ready Cleaning After a Run Error Additional cleaning steps are needed if the run is stopped because of an error If this happens the red Error status light on Sally Sue s or Peggy Sue s front panel will come on see Status Lights on page 12 Click Reset These instructions will display If the troughs in the separation tray are empty click OK and pro...

Page 60: ...paration to open the separation tray 3 Add 800 μL of deionized water to the troughs in the separation tray and let soak for 20 minutes 4 Remove the water by either aspirating with a pipette or with the instrument s vacuum wand 5 Repeat the steps above until the Running Buffer or residues are completely removed 6 To complete the cleaning process select Instrument and click Cleanup Self Test Sally S...

Page 61: ...art a run To view the log when the test is done select Instrument click Properties and View Test Log Viewing and Changing System Properties Select Instrument and click Properties to see system properties Name Location Type Serial number Instrument software version firmware Network name and address Date and time of the instrument clock ...

Page 62: ... name or location click in the name or location boxes and enter the new infor mation To sync the instrument clock with the computer click Set to PC time Viewing Error and Test Logs Select Instrument and click Properties To view a log you can either click View Error Log or View Test Log Insert image here ...

Page 63: ...col Running Stop button We re running an assay The run name time the run started and when it will done show in the progress bar Click Stop to stop the run Cleaning button not active We re running a cleaning protocol The time the cleaning protocol started and when it will be done show in the progress bar Error Reset button We ve detected an error You can go to the Status window in the Run Summary s...

Page 64: ...page 60 Chapter 4 Operating Sally Sue and Peggy Sue Sally Sue and Peggy Sue User Guide ...

Page 65: ...page 61 Sally Sue and Peggy Sue User Guide Chapter 5 Maintenance and Troubleshooting Chapter Overview Software Updates Maintenance Preparation for Storage or Shipment Spare Parts Troubleshooting ...

Page 66: ...e Capillary Discard Tray Empty the capillary discard tray on a daily basis after each run The discard tray is located under the instru ment s sample tray Monthly Ionizer Cleaning Items needed Isopropyl alcohol cleaning swab lint and fiber free VWR P N 89133 806 and the screwdriver P N 040 994 provided in the Sally Sue or Peggy Sue accessory kit 1 Open the instrument s right door 2 Remove the thumb...

Page 67: ...page 63 Sally Sue and Peggy Sue User Guide 3 Using the cleaning swab rub isopropyl alcohol over each of the 4 ionizing pins on the fan to remove any build up 4 Replace the panel and thumbscrew Thumbscrew Pins Pins ...

Page 68: ...nstrument This option allows you to see if the instrument is properly leveled In the Compass for Simple Western main menu select Instrument and click Leveling A screen will display showing the level status NOTE If the reading is outside the 0 05 to 0 05 range you ll need to level the instrument Follow the instructions below or contact ProteinSimple Technical Support or your Customer Service Engine...

Page 69: ... precipitate in a cup you ll need to run the pipet wash before you use the system again NOTES You can follow the recipe below to make your own wash solution or order the Peggy Sue Sally Sue Pipet Wash Solution Kit P N 043 120 It includes Pipet Wash Solution 180 mL and three empty reservoir cups To run the pipet wash you ll need Compass for Simple Western v2 6 7 and up Items needed DTPA Sigma P N 3...

Page 70: ... Place a new sponge in the manifold wash station 3 Open all system trays and remove anything found capillaries capillary boxes cups etc 4 Take three empty clean and dry cups and fill each with 20 mL of the pipet wash solution Place the filled cups in the resource tray 5 Launch Compass for Simple Western Select Instrument in the main menu and click Pipet Wash NOTE To run the pipet wash you ll need ...

Page 71: ...N 7018123 18 gauge and paper towels 1 In Compass for Simple Western select Instrument Manual Clean 2 Fill the 60 mL disposable syringe with 5 Contrad solution and place the tip on the syringe 3 Insert the syringe tip into the first hole on the bottom of the manifold and slowly inject 15 mL of the 5 Contrad solution Repeat this flush for each hole in the manifold 4 Repeat step 3 with DI water 5 Fil...

Page 72: ...pass for Simple Western main menu and click Cleanup Let the cleaning protocol complete this will take about one minute 2 Power down the instrument 3 Open the right and left system doors on Sally Sue or Peggy Sue 4 Wet a cleaning swab with deionized water and clean in between the 12 vertical slots in the gripper 5 Close both doors 6 Turn the instrument power back on System Decontamination You shoul...

Page 73: ...rocedure below 2 Perform the Internal Instrument Cleaning procedure on page 69 3 Perform the Waste Stream Cleaning procedure on page 70 Wipe Down Procedure Items needed 10 bleach solution and towels NOTE You ll need to wear rubber gloves for the wipe down procedure 1 Completely saturate a towel with 10 bleach solution 2 Wipe down the exterior of the instrument 3 Select Instrument in the Compass fo...

Page 74: ...e tip into the first hole and inject 15 mL of the 10 bleach solution Repeat for each manifold hole 4 Repeat step 3 with 5 Contrad solution 5 Repeat step 3 with water 6 Click OK NOTE Contact Protein Simple Technical Support if you have any questions on the manifold cleaning pro cedure Preparation for Storage or Shipment Storage We recommend storing Sally Sue and Peggy Sue if they will not be used f...

Page 75: ... with replacement parts contact ProteinSimple Technical Support at 888 607 9692 Troubleshooting For Sally Sue Peggy Sue or Simple Western assay troubleshooting information please contact ProteinSimple Technical Support at 888 607 9692 option 3 support proteinsimple com or visit http www proteinsim ple com technical_support html You can also contact your local Field Application Specialist for help ...

Page 76: ...page 72 Chapter 5 Maintenance and Troubleshooting Sally Sue and Peggy Sue User Guide ...

Page 77: ...page 73 Sally Sue and Peggy Sue User Guide Chapter 6 General Information Chapter Overview Compliance Safety Guidelines Customer Service and Technical Support Legal Notices ...

Page 78: ...ral requirements CA EN 61326 1 2006 Electrical equipment for measurement control and laboratory use EMC Require ments General requirements EU Safety Guidelines WARNING If Sally Sue and Peggy Sue are not used as specified by ProteinSimple overall safety will be impaired WARNING If Sally Sue or Peggy Sue are damaged and don t function properly stop them safely and contact Pro teinSimple Technical Su...

Page 79: ...ock is engaged When the system status is ready green and the doors are open the robot s system tray Z stage motors and UV power supply are disabled WARNING Do not override the door interlock The interlock protects you from exposure to UV light and a potential pinch hazard that could be caused by moving system trays System Trays CAUTION Don t open or close any of the system trays manually Instead u...

Page 80: ... may present a potential sharps hazard Dispose of used capillaries according to your insti tution s sharps disposal policy Chemical Hazards WARNING CHEMICAL HAZARD Some chemicals used can be potentially hazardous and can cause injury or illness Read and understand the Safety Data Sheets SDSs provided by the chemical manufacturer before you store handle or work with any chemicals or hazardous mater...

Page 81: ...ng the waste bottle Dispose of waste bottle contents in accor dance with good laboratory practices and local state provincial or national environmen tal and health regulations Read and understand the Safety Data Sheets SDSs provided by the manufacturers of the chemicals in the waste container before you store handle or dispose of chemical waste Minimize contact with chemical waste Wear appropriate...

Page 82: ...up when running more than one cycle In Assumes 1 row of sample and 1 row of antibody Waste Origin Size based Assays Volume Trough Reagents for 1 Cycle Matrix Removal Buffer 2 0 mL Wash Buffer 2 0 mL Running Buffer 1 6 mL Water for trough and manifold cleaning 80 0 mL Total waste per cycle 85 6 mL Assay Plate Reagents Separation Matrix 0 240 mL Stacking Matrix 0 240 mL Biotinylated Ladder 0 005 mL ...

Page 83: ...om literature or by calling 888 607 9692 Otherwise call the chemical manufacturer directly or visit their web site Instrument Safety Labels The following safety labels are located on Sally Sue and Peggy Sue Each label displays a safety alert symbol to let you know there s a potential safety hazard Waste Origin Charge based Assays Volume Trough Reagents for 1 Cycle Anolyte 10 mM 1 0 mL Catholyte 10...

Page 84: ...oteinSimple 3001 Orchard Parkway San Jose CA 95134 USA Symbol Description Risk of electric shock Refer to user guide before proceeding Danger of hazardous waste Use caution in these areas This warning only applies if using hazardous material Sally Sue and Peggy Sue reagents are not considered hazardous waste If you are using hazardous materials please contact your field service representa tive to ...

Page 85: ...ict the operation of the Licensed Software to a particular set of conditions Customer shall a limit simultaneous use of the Licensed Software to a maximum of ten 10 Authorized Users and b use the Licensed Software only in connection with the accompanying System purchased by Customer pursuant to the System Quotation and located at the Site 1 2 Authorized User means one 1 User who initiates the exec...

Page 86: ...ary to support Customer s use of the Licensed Software in accordance with this Agreement f shall include any titles trademarks and copyright and restricted rights notices that are included on or in the Licensed Software as delivered by Company on and in any copies of the Licensed Software that it makes and g shall ensure that Customer s use of the Licensed Software does not exceed the scope of the...

Page 87: ...VE ARE EXCLUSIVE AND IN LIEU OF ALL OTHER WARRANTIES WHETHER EXPRESS IMPLIED OR STATUTORY INCLUDING WITHOUT LIMITATION THE IMPLIED WARRANTIES OF MERCHANTABILITY FITNESS FOR A PARTICULAR PURPOSE TITLE AND NONINFRINGEMENT 5 Limitation of Liability NEITHER COMPANY NOR ITS SUPPLIERS SHALL BE RESPONSIBLE OR LIABLE WITH RESPECT TO ANY SUBJECT MATTER OF THIS AGREEMENT OR TERMS OR CONDITIONS RELATED THERE...

Page 88: ... does not agree with the changes to this Agreement Customer must notify Company prior to the effective date of the changes that Customer wishes to terminate its license to the Licensed Software Continued use of the Licensed Software following notice of such changes shall indicate Customer s acknowledgement of such changes and agreement to be bound by the terms and conditions of such changes 7 5 Se...

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