Biotechne ProteinSimple Peggy Sue, User Manual

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Maintenance page 67
Sally Sue and Peggy Sue User Guide
6. If everything is ready, press OK to start the wash protocol. The estimated wash time displays at the top
of the screen:
7. After the wash is complete, remove all three cups, rinse them with distilled water and air dry.
Cleaning the Manifold
Items needed: 5% Contrad 1000 solution (Decon Labs), bleach (diluted 1:10 in DI water), DI water, 60 mL dis-
posable syringe (BD, PN 309653), 45 degree bent, stainless steel dispensing tip, (Nordsen EFD PN 7018123, 18
gauge) and paper towels.
1. In Compass for Simple Western, select Instrument > Manual Clean .
2. Fill the 60 mL disposable syringe with 5% Contrad solution and place the tip on the syringe.
3. Insert the syringe tip into the first hole on the bottom of the manifold and slowly inject ~15 mL of the
5% Contrad solution. Repeat this flush for each hole in the manifold.
4. Repeat step 3 with DI water.
5. Fill the 60 mL syringe with 10% bleach solution and place the tip on the syringe.
6. Insert the syringe tip into the first hole on the bottom of the manifold and slowly inject ~15 mL of the
10% bleach solution. Repeat this flush for each hole in the manifold.
7. Repeat step 6 with DI water.