Biotechne ProteinSimple Peggy Sue, User Manual

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page 70 Chapter 5: Maintenance and Troubleshooting
Sally Sue and Peggy Sue User Guide
Waste Stream Cleaning Procedure
1. Select Instrument from the Compass for Simple Western main menu and click Manual Clean . This will
position the manifold for easy access and turn on the vacuum.
2. Fill the 50-mL disposable syringe with 10% bleach solution. Place the 1-mm tip on the syringe.
3. Flush each hole on the bottom of the manifold. Refer to the picture in “NOTES: You can follow the recipe
below to make your own wash solution, or order the Peggy Sue/Sally Sue Pipet Wash Solution Kit, P/N
043-120. It includes Pipet Wash Solution (180 mL) and three empty reservoir cups. To run the pipet
wash, you’ll need Compass for Simple Western v2.6.7 and up.” on page 65 for proper insertion of the
syringe, then position the syringe tip into the first hole and inject 15 mL of the 10% bleach solution.
Repeat for each manifold hole.
4. Repeat step 3 with 5% Contrad solution.
5. Repeat step 3 with water.
6. Click OK .
NOTE: Contact Protein Simple Technical Support if you have any questions on the manifold cleaning pro-
cedure.
Preparation for Storage or Shipment
Storage
We recommend storing Sally Sue and Peggy Sue if they will not be used for two weeks or longer. To prepare
them for storage:
1. Open the resource tray and remove any remaining capillaries along with the Wash, Running and Matrix
Removal Buffer cups.
2. Discard the unused buffers.
3. Rinse the cups with 0.22-micron filtered deionized water (molecular biology grade or better) and let
them air dry. When dry, cap and store at room temperature.
NOTE: If you don’t remove and cap the reagent cups, this can lead to evaporation and unnecessary con-
centration of reagents which will affect assay performance the next time you run the system.
4. Open the sample tray and remove the sample plate.