Biotechne ProteinSimple Peggy Sue, User Manual

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page 46 Chapter 4: Operating Sally Sue and Peggy Sue
Sally Sue and Peggy Sue User Guide
5. Replace Sponge. Each time you start a new run, you should use a new sponge. Remove and discard
the old sponge and put a new one in the manifold wash station.
6. Start. The resource tray will open. Fill the Wash Buffer, Anolyte and Catholyte cups and insert a capillary
box in the primary position. Add a secondary capillary box if more capillaries are required for you run.
Click
Next when you’re done. The resource tray will close.
NOTES:
Make sure enough capillaries are loaded to complete the run. Insert a capillary box in the primary position
first and if additional capillaries are needed, use the secondary position. When the primary box is empty,
Peggy Sue will automatically move to the secondary box.
Discard any leftover Running Buffer, Wash Buffer and Matrix Removal Buffer in the resource tray cups
before refilling them for new run.