Biotechne ProteinSimple Peggy Sue, User Manual

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Safety Guidelines page 79
Sally Sue and Peggy Sue User Guide
*Assumes 1 row of sample and 1 row of antibody.
Safety Data Sheets
Some chemicals used with Sally Sue and Peggy Sue may be listed as hazardous. Warnings are displayed on
the labels of all chemicals when hazards exist.
SDSs provide you with safety information needed to store, handle, transport and dispose of the chemicals
safely. We recommend updating laboratory SDS records periodically.
Safety Data Sheets for ProteinSimple reagents are available online at www.proteinsimple.com/literature or
by calling (888) 607-9692. Otherwise, call the chemical manufacturer directly or visit their web site.
Instrument Safety Labels
The following safety labels are located on Sally Sue and Peggy Sue. Each label displays a safety alert symbol
to let you know there’s a potential safety hazard.
Waste Origin: Charge-based Assays Volume
Trough Reagents for 1 Cycle
Anolyte (10 mM) 1.0 mL
Catholyte (100 mM) 1.0 mL
Wash Buffer 3.0 mL
Water (for trough and manifold cleaning) 80.0 mL
Total waste per cycle 85.0 mL
Assay Plate Reagents*
Luminol/Peroxide Solution 0.025 mL
Antibodies in Antibody Diluent 0.075 mL
Example Cell Lysate Sample
Cell lysate
Sample Diluent
Premix
Protease inhibitors
Phosphatase inhibitors
Labeled peptides
0.036 mL