Starting a Run: Charge-based Assays (Peggy Sue Only)
page 43
Sally Sue and Peggy Sue User Guide
Starting a Run: Charge-based Assays (Peggy Sue Only)
Step 1 - Get Ready
1.
Create or open your assay file in Compass for Simple Western.
2.
Prepare the instrument: empty waste, refill water and add a new manifold sponge.
3.
Prepare the assay plate using the procedure in the product insert.
IMPORTANT
To prevent well evaporation and get the best results, keep a lid on the assay plate until ready to use.
4.
While the plate is spinning, add Wash Buffer, Anolyte and Catholyte to resource tray cups and insert a
capillary box in the resource tray.
IMPORTANT
Capillaries are light sensitive. Keep the cover on the box until you’re ready to transfer the capillary
box to the resource tray.
5.
Place the assay plate into the sample tray.
6.
Click
Start
.
Step 2 - Start the Run
You can start a run two ways depending on whether you want to run an assay using existing parameters or
set up a new assay.
1.
To start a new run with an existing assay:
Summary of Contents for ProteinSimple Peggy Sue
Page 12: ...page 8 Chapter 2 Getting Your Lab Ready Sally Sue and Peggy Sue User Guide ...
Page 36: ...page 32 Chapter 3 Sally Sue and Peggy Sue Sally Sue and Peggy Sue User Guide ...
Page 64: ...page 60 Chapter 4 Operating Sally Sue and Peggy Sue Sally Sue and Peggy Sue User Guide ...
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