System Tools
Thermo Scientific
DXRxi User Guide
163
9. Bring the scale in the center of the calibration slide into focus.
Close the sample compartment doors.
If you have the optional reflected light brightfield/darkfield illuminator, pull out the
. Then look through the eyepiece while using the focus knob on the stage
controller to bring the edge of the field iris aperture into sharp focus (clockwise knob
rotation moves the stage up). Bringing the aperture into focus also focuses on the
calibration slide.
Figure 101.
Calibration scale in focus at 10x magnification
When the aperture is in focus, push in the field iris control to widen the field of view.
Note
If you have the brightfield only reflected light illuminator (which doesn’t have a
field iris control), bring the scale in the center of the calibration slide (shown in the
image above) into focus instead of the field iris aperture.
Field iris aperture
Each marker is 10 micrometers
Calibration scale
Cross hair
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