Thermo Scientific Orion AquaMate UV-Vis, Руководство пользователя

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Chapter 4
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Water Analysis Test Menu
42 | Orion AquaMate Spectrophotometer User Manual Thermo Scientific
Using the Reverse Color Feature
Reverse color methods use a reagent that, when prepared with samples, deceases in color as
the concentration of the species being measured in the samples increases. Reverse color
methods require the use of both a blank and a reagent blank. The blank is a clear solution
(deionized water) with zero absorbance. The reagent blank is a mixture of the reagent and
deionized water (or initial reagent and sample, as in the zinc by zincon method) and provides a
zero concentration point with the darkest color (highest absorbance). The color of samples
prepared with the reagent will decrease as the concentration increases. The following provides
an overview how to perform a reverse color method.
1. Load the test method in the Water Analysis test menu. The Reverse Color option should be
set to On for the method.
2. Press the Run Test function key.
3. Fill a vial with deionized water. Close the vial tightly with the cap. Wipe the exterior of the
vial.
4. Place the vial into the holder in the sample chamber and close the chamber door.
5. Press the Measure Blank function key to measure the blank.
6. Open the sample chamber door and remove the vial from the holder.