Chapter 9
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Performance Verification
170
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Orion AquaMate Spectrophotometer User Manual
Thermo Scientific
Wavelength Accuracy – Internal
This test locates the peaks of the internal xenon lamp (UV-Vis instruments) or internal
didymium filter (Vis instruments) and displays the expected and measured wavelengths for the
peaks.
•
UV-Vis: A xenon lamp has strong, fundamental lines at 229 nm, 529 nm and 883 nm.
These lines are an essential property of xenon and serve as a fundamental standard.
•
Vis: Didymium glass has strong transmission peaks at 585nm and 807nm. These peaks
are well characterized and suitable for use as wavelength standards.
When running the internal standard test, remember that:
•
The wavelengths and tolerance values are preset and cannot be changed.
•
The vial holder should be empty.
To run the Wavelength Accuracy – Internal test:
1.
Press the test key to access the Test Types menu.
2.
Press the ▲or ▼ key to highlight Performance Verification and press the enter key.
3.
Press the ▲or ▼ key to highlight Wavelength Accuracy - Internal and press the enter key.
4.
Press the Start Test function key.
5.
Once the test is complete, the results will indicate pass or fail for each wavelength.
If the test fails, follow these guidelines:
•
Repeat the test twice to verify that the test is failing consistently.
•
Contact technical support for more troubleshooting advice.
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