Notes about laser intensity
For intensities under 3.5 (PDL) or 35 % (SEPIA II), the pulsed output of the
laser light can be interrupted.
Do not select too high of values on the laser driver (PDL or SEPIA) unless
the laser intensity is not sufficient. High values increase the laser pulse
length.
122
Selecting the Laser
4. Adjust the laser intensity on the laser driver in order to obtain the shortest laser pulse
with respect to measuring instrument response time – "IRF"). In
a second step, adjust the laser intensity on the laser or the laser coupling module (LCU)
(
), to carry out a corresponding adjustment with respect to the correct
fluorescence count rate for FLIM detection.
5. Close the shutter of the continuous wave VIS laser in Beam Path Settings and move all
AOTF sliders to the 0 position.
6. Open the shutter for pulsed lasers by turning the button to
ON
).
7. Activate the desired laser by moving the corresponding slider up all the way (
).
In the control field of the pulsed lasers, the slider setting does not affect the laser intensity.
The laser intensity is controlled outside the LAS AF on the laser driver (PDL800, SEPIA) and
on the laser coupling module.
8. Make sure that the multifunction port (
MFP
,
) is in the right position
Figure 104:
Settings for pulsed VIS lasers in Beam Path Settings
Observe the user manuals provided
Always observe all of the user manuals provided for the individual
components and peripheral devices.
Содержание TCS SP8 SMD
Страница 1: ...10 Living up to Life User Manual Leica TCS SP8 SMD for FCS FLIM and FLCS ...
Страница 4: ...4 Copyright ...
Страница 14: ...14 Contents ...
Страница 18: ...18 Intended Use ...
Страница 20: ...20 Liability and Warranty ...
Страница 28: ...28 General Safety Notes ...
Страница 32: ...32 Additional Notes on Handling the System ...
Страница 44: ...44 System Overview and Properties ...
Страница 60: ...60 SMD Components Figure 31 DSN 102 Dual SPAD Power Supply ...
Страница 80: ...80 Safety Features ...
Страница 102: ...102 Switching On the System ...
Страница 116: ...116 LAS AF ...
Страница 214: ...214 Changing the Specimen ...
Страница 216: ...216 Changing the Objective ...
Страница 218: ...218 Piezo Focus on an Upright Microscope Figure 186 Piezo focus controller Figure 187 Spacer on objective ...
Страница 238: ...238 Switching Off the System ...
Страница 242: ...242 Repairs and Service Work ...
Страница 244: ...244 Maintenance ...
Страница 246: ...246 Disassembly and Transport ...
Страница 248: ...248 Disposal ...
Страница 254: ...254 Contact ...
Страница 256: ...256 Recommended Literature ...
Страница 266: ...266 Appendix Figure 225 Declaration of conformity ...
Страница 268: ...268 Appendix ...
Страница 269: ......