Leica TCS SP8 SMD, Руководство пользователя

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147
FLIM Data Acquisition
Enable the PIE function by clicking on the
2-laser PIE
(
Figure 131
) button.
Select the base frequency in the pull-down menu. The frequency of both lasers is shown as
a
PIE frequency ( Figure 132 ). it is half as high as the base frequency.
Figure 131: Beam Path Settings in LAS AF: Activating exactly two pulsed diode lasers
Figure 132: Dialog for selecting the laser pulse rate in the PIE mode; both lasers pulse at half the base
frequency
Selecting 2-laser PIE is not possible when activating a pulsed diode laser or when selecting
three pulsed diode lasers, and the corresponding button is grayed out ( Figure 133 ).
Figure 133: Activating a pulsed diode laser; the radio button for 2-laser PIE is grayed out
16.2.6 Changing Pulse Frequency for White Light Lasers
If a white light laser has a pulse picker, the repetition rate can be selected via a pull-down
menu in
Beam Path Settings in LAS AF ( Figure 134 ). The laser is at 80 MHz when starting the
system. In the pull-down menu, you can select the following frequencies: 80, 40, 20 and 10
MHz.