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Concentration Measurements—Standard Curve Application

Measuring the Standards for a Standard Curve

Thermo Scientific

GENESYS 10S Vis User Guide

3. Set

Entry concentration

and press

Enter

4. Press

Measure Standards

The

Standards screen

shows the absorbance of each standard, along with the slope,

intercept and correlation coefficient of the standard curve.

To measure standards manually (using Manual 6 or Single Cell Holder)

1. Install the blank and standards.

2. When the parameters are correct, press

Run Standards

3. Set

Entry concentration

4. Press

Measure Blank

If a 6-Position Cell Holder is installed, it moves to the B position to measure the blank
and returns to its previous position.

5. Press

Measure Standards

Summary of Contents for GENESYS 10S Vis

Page 1: ...GENESYS 10S Vis User Guide 269 251700 Revision A October 2009 ...

Page 2: ... Wide Web http www thermo com spectroscopy Thermo Fisher Scientific Inc provides this document to its customers with a product purchase to use in the product operation This document is copyright protected and any reproduction of the whole or any part of this document is strictly prohibited except with the written authorization of Thermo Fisher Scientific Inc The contents of this document are subje...

Page 3: ... Entry 8 Setting Utility Parameters 9 Setting the Date and Time 9 Setting Baseline Expiration Time 11 Setting the Screen Contrast 11 Setting Up the Internal Printer 12 Setting the Utility Parameters for the Printer 12 Chapter 3 Accessories 15 Cell Holders and Cell Holder Accessories 15 Cell Holder Configurations 15 Cell Holder Initialization 18 Changing Cell Holders 18 Installing the 6 Position Ce...

Page 4: ...7 SmartStart 37 Chapter 8 Concentration Units 39 Specifying Concentration Units 39 Creating Custom Units 40 Chapter 9 Calculator Function 41 Chapter 10 Abs and T Measurements Basic A T C 43 Setting the Wavelength 43 Measuring a Blank 44 Measuring Samples 44 Chapter 11 Abs and T Measurements Advanced A T C 45 Recalling a Test 45 Setting Up Test Parameters 46 Taking Measurements 46 Chapter 12 Basic ...

Page 5: ...d Valleys 62 Smoothing Data 63 Determining Peak Height Using a 3 point Net Equation 63 Calculating the Area Under a Curve 64 Viewing and Rescaling Tabular Scan Data 65 Chapter 15 Multiwavelength 67 Recalling a Test 67 Setting Up Test Parameters 68 Adding Wavelengths and Factors 68 Deleting Wavelengths and Factors 69 Taking Measurements 69 Chapter 16 Absorbance Ratio 71 Recalling a Test 71 Setting ...

Page 6: ...etics Results 94 Rescaling and Recalculating Tabular Kinetics Results 97 Chapter 21 Performance Verification 99 Accessing the Performance Verification Tests 99 Troubleshooting Checklist 100 Wavelength Accuracy Internal 101 Wavelength Accuracy Standard 101 Resolution 103 Photometric Accuracy 104 Selecting the Mode 104 Adding Standards 104 Deleting Standards 105 Running the Test 105 Noise 106 Stray ...

Page 7: ... precautionary statements presented in this guide The safety and other special notices appear in boxes Safety and special notices include the following Note Notes contain helpful supplementary information IMPORTANT Follow instructions labeled Important to avoid damaging the system hardware or losing data CAUTION Indicates a hazardous situation which if not avoided could result in minor or moderate...

Page 8: ...Preface viii GENESYS 10S Vis User Guide Thermo Scientific ...

Page 9: ...otometer Components Cell Holders Selecting and Positioning Cuvettes Z dimensions Spectrophotometer Components Here are some major components visible on the outside of a typical instrument Connectors The connectors are on the back of the instrument Keypad Optional printer housing Sample compartment USB port ...

Page 10: ...ide Thermo Scientific WARNING Avoid shock hazard Always turn off the instrument and unplug it from the wall outlet or power strip before you unplug the power cord from the instrument connector USB communication port USB printer port On Off switch A C power connector Fuse compartment ...

Page 11: ...e Keypad Key or button Function Function keys Called Function keys Performs a specific function as displayed above each key Functions will change depending on the software screen Some function keys may not be active Clears the value being entered Returns to the previous screen Deletes the last character entered ...

Page 12: ...lts to the selected printer If PC is selected for the printer sends the method or results to the USB port Displays a menu of software applications Displays the Utility screen Controls the location of the cursor Highlights the value or option for the selection Enters numbers a decimal point and a minus sign for values Cell position keys Selects the cell holder position to be measured B blank and 1 ...

Page 13: ...you press Run Test to start a measurement the instrument attempts to initialize the cell positioner If a single cell holder is installed the message Error Single Cell Holder found Use Single Cell Holder appears Press Accept Change to continue the measurement with a single cell or install the 6 cell changer and press Cancel Change Cell Type Wavelength Optical Glass Borosilicate Glass 360 nm to 1100...

Page 14: ...ll or cuvette for your blank and your samples Z dimensions The figure below illustrates the position of the light beam in the instrument Beam size specifications are shown below Distance from bottom of cuvette to center of beam Z dimension 8 5 mm Beam dimensions 2 mm wide by 7 mm tall Note The pathlength of test tubes is not as well defined as that of square cuvettes Note Always place test tubes i...

Page 15: ...r Entering Parameter Values The following sections describe the use of the keypad to interact with menus and controls These sections provide instructions for Numeric Entry Menu Selection On Off Toggle Alphanumeric Entry Numeric Entry With the parameter e g Wavelength highlighted start typing the numeric value An Entry window with the value range appears Type the complete entry and press Enter ...

Page 16: ...ith the parameter Units or Sample Positioner highlighted press Enter to display the selection list Highlight the appropriate item and press Enter On Off Toggle With the parameter e g AutoPrint highlighted press Enter to toggle to the opposite value Alphanumeric Entry With the parameter e g Test Name highlighted press Enter The Name Entry screen appears Highlight the desired character and press Add...

Page 17: ...setting screen contrast settings and printer setup You can also access a directory of all stored tests and the calculator function You cannot set utility parameters or change the utility when the instrument is carrying out a measurement Press Utility on the keypad Setting the Date and Time Highlight Date Time Setup and press Enter You can modify the date time format and time Y To set the date 1 Hi...

Page 18: ... the time 1 Highlight Set Time and press Enter 2 To set the hour press Set Hour type in the hour and press Enter 3 To set the minutes press Set Minute type in the minute and press Enter 4 To select between AM and PM if in AM PM time format press Set AM PM until the appropriate setting appears Since the tungsten lamp of the GENESYS 10S Vis spectrophotometer requires a 30 minute warm up the spectrop...

Page 19: ...ts are made in a production setting across multiple shifts or when the nature of the blank sample changes dramatically with time Y To set the baseline expiration time 1 Highlight Baseline Expiration hr min and press Enter 2 Enter the desired time in the Entry baseline expiration time field and press Enter Setting the Screen Contrast To make it easier to read the display you can adjust the screen c...

Page 20: ...rinter See Loading Paper in the Internal Printer on page 22 in Accessories for instructions on installing the printer Setting the Utility Parameters for the Printer Paper printouts are available from both the internal printer and a USB printer attached to the instrument Alternatively displayed ASCII text and graphics can be sent to a computer using a USB connection Enabling the PC as the printing ...

Page 21: ...S 10S Vis User Guide 13 3 Select the printer and press Enter until On appears 4 Press Esc to save the settings Note Text and graphics can be output via the internal printer and an external printer on the USB port Only text no graphics can be output via the USB connection to a computer ...

Page 22: ...2 Setting Up the Instrument Setting Up the Internal Printer 14 GENESYS 10S Vis User Guide Thermo Scientific ...

Page 23: ...tching off the instrument Cell Holders and Cell Holder Accessories The instrument is shipped with both the 6 Position Cell Holder installed at the factory and the Single Cell Holder Directions to remove and install these cell holders and other cell holder accessories are below Cell Holder Configurations The following table shows the available cell holders and their accessories You can install or r...

Page 24: ...lator Temperature Control Must be installed in the B 2 and 4 positions Test Tube Holder Must be installed in the B 2 and 4 positions 50 mm Rectangular Long Pathlength Cell Holder Must be installed in the B 2 and 4 positions 50 mm Cylindrical Long Pathlength Cell Holder Must be installed in the B 2 and 4 positions Cell Changer System Single Cell System ...

Page 25: ...ell Holder Cannot use 100 mm path cells with Cell Positioner 100 mm Cylindrical Long Pathlength Cell Holder Cannot use 100 mm path cells with Cell Positioner Thin Film Filter Holders Must be installed in the B 2 and 4 positions Adjustable Filter Lens Holder Must be installed in the B 2 and 4 positions Cell Changer System Single Cell System ...

Page 26: ...lindrical or rectangular Combination Systems Must be installed in the B 2 and 4 positions Not applicable Cell Changer System Single Cell System Note If while in this screen you remove the cell changer and press Run Sample the instrument will display Fatal Error 8 Press Esc to return to main menu Pressing Esc returns you to the parameter menu screen of the test Press Run Test and the instrument wil...

Page 27: ...led in the spectrophotometer can easily be removed to install other accessory cell holders See Removing the 6 Position Cell Holder and the Single Cell Holder on page 20 Installing the 6 Position Cell Holder and the Single Cell Holder Y To install the 6 Position Cell Holder and the Single Cell Holder 1 Open the sample compartment door and let it rest on its hinge 2 With one hand carefully lower the...

Page 28: ...d the Single Cell Holder 1 Open the sample compartment door and let it rest on its hinge 2 With one hand loosen the captive thumbscrew 3 With your other hand pull straight up on the cell holder and lift it out of the sample compartment 4 Close the sample compartment door Installing Accessory Cell Holders Make sure that you have the correct cell holder baseplate installed Note If the cell holder is...

Page 29: ...he new cell holder into the appropriate position and secure it to the baseplate by tightening the captive screw See Installing the 6 Position Cell Holder and the Single Cell Holder on page 19 for instructions on installing the complete accessory assembly in your instrument Installing the Internal Printer Optional Note To use 100 mm long pathlength cells you must install the Single Cell Holder base...

Page 30: ... 6 Lower the hinge so it is out of the way 7 Connect the wires and press into place with a small screwdriver There is only one way that the connectors will fit Each connector has a slight D shape Make sure the side of the connector with the shiny metal contacts faces away from the printer and towards the plastic door 8 Use the clip on the hinge to secure the wires 9 Install the printer door by sli...

Page 31: ...esktop printers supporting HP PCL 5 0 format and later To print to an HP PCL printer connect the USB cable to the printer and to the USB port on the back of the instrument see Connectors on page 1 Note Make sure the paper roll holders are in place as shown When installed correctly they fit flush with the top of the instrument Note Arrows on the paper roll holders indicate the direction of the pape...

Page 32: ...3 Accessories External Printers 24 GENESYS 10S Vis User Guide Thermo Scientific ...

Page 33: ...thout changing cuvettes in the cell changer The instrument automatically measures the blank and advances the cell changer to measure the samples in positions 2 and 4 Single Cell Holder Place the blank in the cell holder measure it place a sample in the cell holder and then measure your sample This process is completely manual The cell position buttons do not function when you select Single Cell Ho...

Page 34: ...d your samples in the other cell positions Regardless of where the cell holder is positioned when you press Measure Blank the cell holder automatically goes to the blank position and measures the blank However you can use the cell position buttons to select a different position for the measurement Note When you have the Cell Changer installed the instrument always considers the material in the B p...

Page 35: ...and Positioning Cuvettes on page 5 Place the blank cuvette in Cell B of the cell changer Cell Correction Y To run Cell Correction 1 Load the test type or stored test 2 If Cell Correction is not visible highlight More parameters and press Enter Note Cell Correction is not active from the Main Basic ATC screen Note Cell Correction is active only when the 6 Position Cell Holder is set to either Auto ...

Page 36: ...ple cell for the range of wavelengths you specify in Scanning mode Discrete nms Cell Correction is run on a blank and up to five sample cells for up to 31 user specified discrete wavelengths 6 If you selected Scan mode in the preceding step specify the Start Wavelength and the Stop Wavelength values 7 Press Run Corr to start Cell Correction Note When Cell Correction is activated additional paramet...

Page 37: ...ctors which then are automatically applied during all subsequent tests if Cell Correction is activated Specifying Wavelengths for Discrete nms Mode Y To specify wavelengths for Discrete nms mode 1 Highlight Sample Positioner and press Enter to set this parameter to either Auto 3 when using three large cell holders or Auto 6 when using six small cell holders 2 Highlight Number of Matched Cuvettes a...

Page 38: ...ering all the wavelengths press Run Corr to start Cell Correction The application measures the other cells against the blank and records stores and dates the measurements From these measurements the application establishes the required correction factors which are applied during all subsequent tests if Cell Correction is activated Note Match cells at all analytical wavelengths Matching at one does...

Page 39: ... for all parameters are the same as when the instrument was last used If you load a saved test the values for all parameters stored with it replace the current values of the test parameters Software Password This password lets you lock test setups test parameters so they may not be overwritten or deleted The password also lets you remove the security so you may edit the test parameters See the Loc...

Page 40: ...the name 2 Highlight the first character for the test name and press Add Character 3 Continue selecting and adding characters until the name is complete 4 Press Accept Name Saving a Test After a method has been configured there are two options for saving a test The test method can be saved to an external memory device using the front USB port or to the internal memory of the spectrophotometer ...

Page 41: ...entific GENESYS 10S Vis User Guide 33 Y To save a test in the instrument library 1 Press Save Test A prompt appears 2 Select the appropriate location and press Enter 3 Create or enter a name for the test Use the procedure in Naming a Test on page 31 ...

Page 42: ...cluded as a SmartStart test See SmartStart on page 37 6 Highlight the appropriate SmartStart option and press Enter to save the test Loading Test Files You can load saved test files from the internal memory from the Utility screen Y To load test files 1 To access all test files press Utility 2 Highlight Stored Tests Directory and press Enter ...

Page 43: ... it and press Lock Unlock Enter the password and press Enter Deleting a Test To delete a test highlight it and press Delete Test Note To view the stored tests of a particular test type press Test select a test type and press Stored Tests Note To lock or unlock access to the file you must enter the software password of this manual ...

Page 44: ...6 Managing Stored Tests Deleting a Test 36 GENESYS 10S Vis User Guide Thermo Scientific ...

Page 45: ...is test and prepares for immediate measurement If you select more than one test as SmartStart tests the instrument when powered on automatically displays a menu containing only those tests Y To set up a single test SmartStart 1 Press Utility to display the Utility screen 2 Highlight Stored Tests Directory and press Enter 3 Highlight the appropriate test and press Select Test An arrow sign indicate...

Page 46: ...nu will be displayed upon power up Y To set up a multiple test SmartStart 1 Follow steps 1 through 3 in the procedure above for setting up a single test SmartStart 2 Select the desired tests An arrow sign indicates the tests selected for the SmartStart menu Press Esc to return to the Utility screen or power down the instrument Note To remove tests from the SmartStart menu see the procedure above f...

Page 47: ... include a parameter for units which labels the results The spectrophotometer includes a set of basic concentration units All programs in the spectrophotometer use the same list of basic units C concentration μg L ppm M L ppb mM L g L IU mg L pM μL mg mL ng μL Custom units can also be created for more information see Creating Custom Units on page 40 Y To select the units 1 Highlight Units and pres...

Page 48: ... list This custom concentration unit can be changed when desired Y To create custom units 1 With the Units Selection window displayed press Edit Unit Use this screen to Delete the name of a unit Delete a character in the name of a unit Add a character to the name of a unit Accept the name of a unit 2 Follow steps 2 through 4 in Naming a Test on page 31 The new custom unit appears in the list of ba...

Page 49: ...ator function 1 From the Utility menu highlight Calculator and press Enter 2 Use the numeric keypad to enter the desired value 3 Press the desired function x or 4 Enter the second desired value and press Enter Note You can only add subtract multiply or divide two lines of numbers at a time ...

Page 50: ...9 Calculator Function 42 GENESYS 10S Vis User Guide Thermo Scientific ...

Page 51: ...tion the result appears along with the type of measurement the date and time the wavelength and the cell position used for the measurement To toggle between Absorbance Transmittance and Concentration press Change Mode You can toggle modes whenever you see Change Mode When Basic ATC is set to Absorbance or Transmittance these capabilities are provided Setting the Wavelength Measuring a Blank Measur...

Page 52: ...nsmittance value for the blank press a number key and enter the desired value in the Entry field 3 Press Measure Blank Measuring Samples If a 6 Position Cell Changer is installed place the samples in the cell positions and press the corresponding cell position button to move the cell holder to the measuring position The absorbance ABS or percent transmittance T measurement appears on the display I...

Page 53: ... the measurement of multiple samples with a cell changer This section covers Selecting the measurement mode Absorbance or Transmittance Cell Correction Recalling a Test Setting Up Test Parameters Taking Measurements To get started press Test highlight Advanced A T C and press Enter Recalling a Test Y To recall a test 1 In the Advanced A T C screen press Stored Tests 2 Highlight the test you want t...

Page 54: ...2 When the parameters are set press Save Test to save the test or Measure Sample to measure a blank or samples Taking Measurements Y To take measurements automatically using Auto 6 or Auto 3 1 Press Run Sample 2 Install the blank and samples 3 Press Measure Sample Y To take measurements manually using Manual 6 or Single Cell Holder 1 Press Run Sample 2 When prompted place the blank and samples int...

Page 55: ...king Measurements Thermo Scientific GENESYS 10S Vis User Guide 47 The sample measurement appears If a 6 Position Cell Holder is installed press the cell position buttons to reposition the cell holder and measure the rest of the samples manually ...

Page 56: ...11 Abs and T Measurements Advanced A T C Taking Measurements 48 GENESYS 10S Vis User Guide Thermo Scientific ...

Page 57: ...er accuracy and the ability to save and recall methods and data use the Standard Curve application mode described in Concentration Measurements Standard Curve Application on page 83 Measuring concentration using the Basic ATC mode is similar to measuring Absorbance or T Basic ATC mode lets you measure concentration using either a factor or one standard to convert absorbance readings to concentrati...

Page 58: ...Using Conc Std to Measure Concentration In this mode a standard sample of well known concentration is used to determine the concentration of samples The concentration of unknown samples is determined ratiometrically from the measured standard The measurement can be expressed mathematically as where the standard concentration is precisely known the Abs T of the standard and the Abs T of the unknown...

Page 59: ... the absorbance of the standard and displays the absorbance and calculated factor Using Conc Factor to Measure Concentration In this mode a concentration factor is used to determine the concentration of samples The concentration of unknown samples is determined ratiometrically from the entered factor The measurement can be expressed mathematically as Abs T of Sample Factor Concentration of Sample ...

Page 60: ...e unit in the list and press Enter 8 Press Esc to return to the Conc With Factor screen Measuring Samples If the 6 Position Cell Changer is installed place the sample you want to measure in one of the cell positions and press the corresponding cell position button to move the cell changer to the measuring position The measurement appears If the Single Cell Changer is installed remove the blank and...

Page 61: ...th a factor Recalling a Test Setting Up Test Parameters Measuring a Standard or Entering a Factor only if you select either concentration with one standard or concentration with a factor Measuring a Blank Measuring Samples To get started press Test highlight Advanced A T C and press Enter Recalling a Test Y To recall a test 1 Press Stored Tests 2 Highlight the test and press Enter or Load Test to ...

Page 62: ...the test or Run Standard to measure a standard if in Conc Std or press Run Test if in Conc Factor Measuring a Standard Y To measure a standard automatically using Auto 6 or Auto 3 1 With Measurement Mode set to Conc Std press Run Standard 2 Enter the concentration of the standard and press Enter 3 Press Measure Blank 4 Insert the blank and standard 5 Press Enter to measure the blank and samples an...

Page 63: ...Y To measure a sample automatically using Auto 6 or Auto 3 1 Press Run Test 2 When prompted place the blank and samples in their cell positions and press Enter The instrument measures the blank and samples and displays the sample measurements 3 Press Measure Sample to measure additional samples Y To measure samples manually using Manual 6 or Single Cell Holder 1 Press Run Test 2 Insert the blank a...

Page 64: ...13 Concentration Measurements Advanced A T C Measuring Samples 56 GENESYS 10S Vis User Guide Thermo Scientific ...

Page 65: ...Manipulating Scan Data Rescaling Graphical Scan Data Determining Peak Height Using a 3 point Net Equation Calculating the Area Under a Curve Labeling Peaks and Valleys To get started press Test highlight Scanning and press Enter Recalling a Test Y To recall a test 1 Press Stored Tests 2 Highlight the test you want to recall and press Enter Note The Scanning application lets you measure only one sa...

Page 66: ...to save the test or Run Test to measure a blank or a sample Collecting a Baseline Scan Y To collect a baseline scan 1 Press Run Test 2 Place the blank in the B position Note If Cell Correction is ON you must run the Setup Correction application before you can access Run Test or Measure Samples Note If Auto Save Data is ON you must enter a Data File Name before you can access Run Test or Measure Sa...

Page 67: ...ss Run Test If a 6 Position Cell Holder is installed place the sample in cell position 1 2 Press Measure Sample Note To switch between tabular and graphical displays press Graph Tabular Note If a 6 Position Cell Holder is installed be sure to place the sample in cell position 1 The instrument always uses cell position 1 to scan the sample Note To switch between tabular and graphical displays press...

Page 68: ...s on the Scan Data Labeling Peaks and Valleys Rescaling Graphical Scan Data You can modify the scale of your scan data plot automatically or manually When you select Auto Scale the instrument scales the X and Y axes so all the data appears on the plot When you select Manual Scale you select specific minimum and maximum values for the axes When you modify the scale the instrument recalculates and d...

Page 69: ...pears on the plot Y To use the Cursor 1 With your scan data displayed on the edit scale screen press Cursor 2 Use Cursor to move to the desired minimum wavelength value Press Set Min X to redraw the plot using the new minimum wavelength value 3 Repeat using Cursor and Set Max X to set the new maximum wavelength Y To use the Manual Scale function 1 Press Manual Scale to display the manual scale opt...

Page 70: ...um values Performing Calculations on the Scan Data You can modify your graph by performing calculations on the data In the Edit Graph screen press Math The Math screen provides these capabilities Labeling Peaks and Valleys Smoothing Data Calculating the Area Under a Curve Determining Peak Height Using a 3 point Net Equation Labeling Peaks and Valleys Y To label valleys and peaks 1 With your scan d...

Page 71: ...noise you can smooth the data with the smoothing function Y To smooth data With your scan data displayed on the edit graph screen press Smoothing On Determining Peak Height Using a 3 point Net Equation Y To determine 3 point net measurements 1 With your scan data displayed on the edit graph screen press Math 2 Press 3 Pt Net The 3 point net measurement screen shows the cursor options and three cur...

Page 72: ...er and right cursor lines Select the wavelengths with Cursor and Cursor Repeat until all three wavelengths have been selected 5 Press Enter Factor to access the set factor box Enter the desired factor and press Enter The instrument calculates the value for the 3 point net absorbance for the selected wavelengths multiplied by the selected factor Calculating the Area Under a Curve Y To calculate the...

Page 73: ...or line Select the wavelength with Cursor and Cursor 5 Press Set Options to access the set options window 6 Highlight Factor Enter the desired factor and press Enter 7 Highlight Calculation baseline 8 Press Enter to toggle between Zero and Tangent 9 Press Esc to return to the area under a curve screen The instrument calculates the area under a curve for the selected wavelengths factor and calculat...

Page 74: ...a displayed on the edit screen press Use All Data Y To select specific start and end wavelengths 1 With your table of scan data displayed on the edit screen highlight the appropriate data point in the table 2 Press Start nm or End nm The instrument highlights the selected data points To display the plot using the highlighted data points press Graph ...

Page 75: ...elengths of interest are well known Use Multiwavelength for Recalling a Test Setting Up Test Parameters Cell Correction Adding Wavelengths and Factors Deleting Wavelengths and Factors Taking Measurements To get started press Test highlight Multiwavelength and press Enter Recalling a Test Y To recall a test 1 Press Stored Tests 2 Highlight the test to recall and press Enter to display its parameter...

Page 76: ...d factors If you have previously selected the wavelengths to measure press Save Test to save the test or Run Test to measure a blank or samples If you have not selected the wavelengths you can add wavelengths and factors as shown below Adding Wavelengths and Factors Y To add wavelengths and factors 1 Press Set nms Note If Cell Correction is ON you must run the Setup Correction application before y...

Page 77: ... and factors Deleting Wavelengths and Factors Y To delete wavelengths and factors 1 Press Set nms 2 Highlight the first wavelength and factor pair to delete 3 Press Delete nm Taking Measurements You can access Multiwavelength acquisition from either the Set nms screen shown above or from the Multiwavelength setup screen Y To take measurements automatically Auto 6 or Auto 3 1 Press Run Test to disp...

Page 78: ...he B position The holder can hold five samples 3 Press Measure Blank If a 6 Position Cell Holder is installed it moves to the B position to measure the blank and returns to its previous position 4 With the list of wavelengths and factors displayed press Measure Samples to measure and display the absorbance at each wavelength If you set the measurement mode to Concentration Factor the calculated co...

Page 79: ...ects of a sample matrix Typically used in quality control applications an absorbance ratio provides a convenient and quick diagnostic test for sample quality Use Absorbance Ratio for Recalling a Test Setting Up Test Parameters Cell Correction Measuring a Blank Measuring Samples To get started press Test highlight Absorbance Ratio and press Enter Recalling a Test Y To recall a test 1 In the Absorba...

Page 80: ...test parameters 1 In the Absorbance Ratio screen highlight the desired parameter 2 When the parameters are set press Save Test to save the test or Run Test to measure a blank or samples Measuring Samples Y To measure samples automatically using Auto 6 or Auto 3 1 Install the blank and samples Note If Cell Correction is ON you must run the Setup Correction application before you can access Run Test...

Page 81: ... Run Test 2 Install the blank and sample A 6 Position Cell Holder can hold five samples 3 Press Measure Blank If a 6 Position Cell Holder is installed it moves to the B position to measure the blank and returns to its previous position 4 Press Measure Sample If a 6 Position Cell Holder is installed press the cell position buttons to reposition the cell holder and measure the rest of the samples ma...

Page 82: ...16 Absorbance Ratio Measuring Samples 74 GENESYS 10S Vis User Guide Thermo Scientific ...

Page 83: ...ally used in quality control applications an absorbance difference application provides a convenient and quick diagnostic test for sample quality Use Absorbance Difference for Recalling a Test Setting Up Test Parameters Cell Correction Measuring a Blank Measuring Samples To get started press Test highlight Absorbance Difference and press Enter Recalling a Test Y To recall a test 1 In the Absorbanc...

Page 84: ...asuring a Blank Measuring Samples Setting Up Test Parameters Y To set up test parameters 1 In the Absorbance Ratio screen highlight the parameter to set 2 When the parameters are set press Save Test to save the test or Run Test to measure a blank or samples Note If Cell Correction is ON you must run the Setup Correction application before you can access Run Test or Measure Samples Note If Auto Sav...

Page 85: ...press Run Test 2 Install the blank and samples 3 Press Enter Y To measure samples manually using Manual 6 or Single Cell Holder 1 In the Absorbance Difference screen press Run Test 2 Install the blank and samples A 6 Position Cell Holder can hold five samples 3 Press Measure Blank If a 6 Position Cell Holder is installed it moves to the B position to measure the blank and returns to its previous p...

Page 86: ...asuring Samples 78 GENESYS 10S Vis User Guide Thermo Scientific 4 Press Measure Sample If a 6 Position Cell Holder is installed press the position buttons to reposition the cell holder and measure the rest of the samples manually ...

Page 87: ...e multiplied by the measured peak height to give the concentration of the measured analyte in the appropriate concentration units Use 3 Point Net for Recalling a Test Setting Up Test Parameters Cell Correction Taking Measurements To get started press Test highlight 3 Point Net and press Enter Note If Cell Correction is ON you must run the Setup Correction application before you can access Run Test...

Page 88: ...up test parameters 1 Highlight the desired parameter 2 When the parameters are set press Save Test to save the test or Run Test to measure a blank or samples Taking Measurements Y To take measurements automatically Auto 6 or Auto 3 1 With the 3 Point Net Setup screen displayed and the parameters set press Run Test to display the 3 Point Net measurement screen Note If Cell Correction is ON you must...

Page 89: ... and samples If a 6 Position Cell Holder is installed place the blank in the B position The holder can hold five samples 3 Press Measure Blank If a 6 Position Cell Holder is installed it moves to the B position to measure the blank and returns to its cell position 4 Press Measure Sample If a 6 Position Cell Holder is installed press the cell position buttons to reposition the cell holder and measu...

Page 90: ...18 3 Point Net Taking Measurements 82 GENESYS 10S Vis User Guide Thermo Scientific ...

Page 91: ...ve is used to measure the concentration of samples Use Standard Curve for Creating a standard curve set up the parameters and then measure the standards for the curve Cell Correction Measuring Samples Viewing calibration curve data select between graphical and tabular displays Editing a Standard Curve change the number of standards select a different curve fit or delete points from the curve Viewi...

Page 92: ...itions 2 Set parameters for measuring the standards See Parameters on page 117 for a complete list a Enter the Test Name Wavelength Reference Wavelength Correction and Reference Wavelength b Select the Curve Fit Units and Sample Positioner c Set Number of Standards and Number of Samples d Enter the low and high limits e Select the settings for Statistics and AutoPrint f Run Cell Correction Measuri...

Page 93: ...nce of each standard along with the slope intercept and correlation coefficient of the standard curve Y To measure standards manually using Manual 6 or Single Cell Holder 1 Install the blank and standards 2 When the parameters are correct press Run Standards 3 Set Entry concentration 4 Press Measure Blank If a 6 Position Cell Holder is installed it moves to the B position to measure the blank and ...

Page 94: ...elation coefficient of the standard curve Using the Standards Screen Here is an example of the Standards screen showing measurement results You can use this screen for Displaying a graph of the standard curve data press View Graph Saving a Test press Save Test Editing a Standard Curve press Edit Standards Measuring Samples press Run Test Measuring Samples Y To measure samples automatically using t...

Page 95: ... the blank and returns to its previous position 4 Press Measure Samples If a 6 Position Cell Holder is installed press the cell position buttons to reposition the cell holder and measure the rest of the samples manually When the instrument has measured all the samples the Standards screen shows the absorbance and concentration of each sample Editing a Standard Curve You may edit the concentration ...

Page 96: ...alue in the Entry field 5 Press Enter Y To add a standard 1 With the standard curve displayed press Edit Standards 2 Highlight Add Standard 3 Enter the concentration value of the additional standard in the Entry field 4 Press Enter 5 Press Measure Standards to remeasure all the standards Y To delete a standard 1 With the standard curve displayed highlight the standard to delete and press Edit Stan...

Page 97: ...andards and press Enter All standards and measurements are removed from the screen Y To select a different curve fit for a standard curve 1 With the standard curve you want to edit displayed as a graph press Change Fit 2 Highlight the curve fit to use for the standard curve and press Enter The instrument applies the selected curve fit to the data and displays the new fit Note To change the curve f...

Page 98: ...19 Concentration Measurements Standard Curve Application Editing a Standard Curve 90 GENESYS 10S Vis User Guide Thermo Scientific ...

Page 99: ...it of the embedded software Software control streamlines the use of more sophisticated computer applications to analyze kinetics data after collection Use Kinetics for Recalling a Test Setting Up Test Parameters Cell Correction Measuring a Blank Measuring Samples Recalling and Recalculating Graphical Kinetics Results Rescaling and Recalculating Tabular Kinetics Results Modifying the scale of the p...

Page 100: ...tored Tests 2 Highlight the test to recall and press Enter This screen provides these capabilities Setting Up Test Parameters Setting up Cell Correction Saving a Test Viewing the list of stored tests Measuring a Blank Measuring Samples Setting Up Test Parameters Y To set up test parameters 1 In the Kinetics screen highlight the desired parameter ...

Page 101: ...m Test Initiation to first measurement allows for sample equilibration Adv Interval Time Enters the time between repeated readings Measure Blank as function key Selects the frequency of zeroing the instrument Once or Every Reading Note If Cell Correction is ON you must run the Setup Correction application before you can access Run Test or Measure Samples Note If Auto Save Data is ON you must enter...

Page 102: ...n a graphical display you can press Edit Graph and then press Cursor to move the cursor line from one position to another on the plot As the cursor moves the rate and result values indicate the values for the point where the cursor is located Recalling and Recalculating Graphical Kinetics Results The Kinetics application lets you view and manipulate results in graphical or tabular form When your r...

Page 103: ...e edit screen lets you Use Auto Scale to change the scale display the new graph and recalculate the results Use Manual Scale to change the scale display the new graph and recalculate the results Use the cursor to select new minimum or maximum values for the X axis and recalculate the results Y To use Auto Scale With your kinetics data displayed on the Edit Graph screen press Auto Scale The instrum...

Page 104: ...d maximum values and displays the recalculated rate and result 3 Continue until you have entered all the values you want to change Y To use the Cursor function 1 With your kinetics data displayed on the edit screen press Cursor to display the cursor options 2 Press Cursor or Cursor to position the cursor line on the appropriate point on the graph The data for the selected point appears 3 When the ...

Page 105: ...en you modify the start and end times or select all the data the instrument recalculates and displays the new reaction rate and result The edit screen lets you Use all the data to recalculate the results Select specific start and end times for the rate calculation and recalculate the results Y To use all the data to calculate the reaction rate With your table of kinetics data displayed on the edit...

Page 106: ...20 Kinetics Rescaling and Recalculating Tabular Kinetics Results 98 GENESYS 10S Vis User Guide Thermo Scientific ...

Page 107: ...Printer Test Run the appropriate tests regularly and maintain a log of results to help document the reliability of the instrument and indicate potential performance issues Accessing the Performance Verification Tests Y To access the Performance Verification tests 1 Press Tests 2 Highlight Performance Verification and press Enter Note If a printer is installed and turned on the instrument automatic...

Page 108: ...ilters and standards are clean The sample compartment door is closed during the test The sample compartment is clear of obstructions The cell holder assembly is installed properly If the 6 Position Cell Holder is installed run the test once with the sample compartment door open to verify that the 6 Position Cell Holder is moving smoothly No problems are indicated by the power on diagnostics after ...

Page 109: ...curacy Internal test 1 Highlight Wavelength Accuracy Internal and press Enter 2 Press Start Test The results indicate pass or fail for each wavelength If the test fails follow these guidelines Repeat the test twice to verify that the test is failing consistently Contact technical support for more troubleshooting advice Wavelength Accuracy Standard This test measures the absorbance of a wavelength ...

Page 110: ...ength standard at only five wavelengths Y To run the Wavelength Accuracy Standards test Highlight Wavelength Accuracy Standards and press Enter Y To add a wavelength 1 Press Add nm and enter the wavelength value in the Entry field 2 Press Add nm again to add the wavelength to the list 3 Enter the tolerance for the entered wavelength in the Entry field 4 Press Enter Y To delete a wavelength 1 Highl...

Page 111: ...jacent features in a spectrum The test is performed using a 0 02 v v solution of toluene in hexane and requires a hexane blank When running the internal standard test remember that the wavelengths and tolerance values are preset and cannot be changed Y To run the Resolution test 1 Highlight Resolution and press Enter Ensure that hexane is in the blank position and toluene in hexane is in cell 1 2 ...

Page 112: ...tric Accuracy standards calibrated to known absorbance values at specified wavelengths Measure the standards in the order they are listed on the test screen You can use one to five standards Y To display the Photometric Accuracy screen 1 Highlight Photometric Accuracy 2 Press Enter Selecting the Mode To switch between absorbance and Transmittance press Change to T or Change to ABS until the approp...

Page 113: ...erance for the entered wavelength in the Entry field 6 Press Enter The test screen displays the values you just entered for that standard 7 Press Start Test to begin the measurement or Press Esc to save the test Deleting Standards Y To delete a standard 1 Highlight the appropriate standard 2 Press Delete Std Running the Test In the Photometric Accuracy screen make sure the wavelengths absorbance o...

Page 114: ...m All test parameters are determined by instrument specifications and cannot be changed by the user When running the noise test remember Perform the 0A measurement with the cell holder empty Optionally you can perform the 2A measurement with a 2A filter Y To run the Noise Measurement test 1 Highlight Noise Measurement 2 Press Enter 3 With the Blank position empty insert the 2A filter in position 1...

Page 115: ...velength of interest Position B should be empty Use position 1 for the 220 nm stray light standard SRE 220 or equivalent Use position 2 for the 340 nm stray light standard SRE 340 or equivalent Use position 3 for the 400 nm stray light standard SRE 400 or equivalent Running the Test In the Stray Light screen make sure the wavelengths and tolerances are set correctly Y To run the Stray Light test 1...

Page 116: ...on screen highlight Internal Printer Test 3 Press Enter 4 Press Start Test You can press Stop Test to stop the test The test print routine appears on the printed results If the test fails follow these guidelines Make sure the internal printer is the selected as the printer device on the Utility screen Make sure the internal printer is installed correctly Return to the main screen and press Enter I...

Page 117: ... including the keypad with a soft cloth to remove any dust or spills Water isopropyl alcohol and other common laboratory cleaning agents may be used if necessary Always clean up spills as soon as they occur to prevent or minimize damage to the instrument If concentrated acids or bases or any hydrocarbon materials are spilled on the instrument clean up the affected area immediately WARNING Operatin...

Page 118: ...e cell Cleaning methods depend to some extent on the nature of the contaminating material It is important to identify the residual material in the cell that needs to be removed Refer to the following table for suggestions on cleaning methods solvents and material Solvents Examples Suggested Cleaning Methods Aqueous Protein Biologics DNA Warm water with detergent Dilute nitric acid 10 rinse Copious...

Page 119: ...ganic solvent Warm water with detergent Dilute nitric acid 10 rinse Copious water rinse IMPORTANT Keeping the cell clean is very important for long cell life Term Definition Dilute acid Dilute nitric acid 10 Acid Hydrochloric 5M acid or nitric acid 5M see the Note below Solvent rinse Rinse with the solvent that was originally used to solvate your analyte Copious water rinse Use a pure water e g de...

Page 120: ...too much pressure or the surface of the windows may be damaged Be sure to remove all fingerprints Changing the Fuse The fuse is located in the power entry module located at the center of the back panel of the instrument 120 VAC 2 5 A Slo Blo 240 VAC 1 25 A Slo Blo 2 required Y To change the fuse 1 Turn off and unplug the instrument from the wall outlet or power strip 2 Position the instrument so y...

Page 121: ...ng them in so they snap into place 8 Replace the fuse cover 9 Replace the power cord 10 Plug the instrument back in to the wall outlet or power strip and turn on the power Replacing the Tungsten Lamp Note If the fuse blows again contact your distributor or technical support WARNING This lamp gets very hot during operation Before removing the lamp turn off the instrument and allow the lamp to cool ...

Page 122: ...urn 4 Raise the lamp door 5 Unscrew the thumbscrew while holding the lamp assembly in place 6 Carefully pull the assembly out until you can reach the connector 7 Release the tab and disconnect the cable 8 Unpack the new lamp assembly 9 Use a soft lint free cloth to wipe off any fingerprints on the lamp 10 Connect the cable to the connector WARNING Turn off and unplug the instrument before removing...

Page 123: ...1 Position the new assembly as shown above making sure to align the pins correctly 12 Tighten the thumbscrew 13 Close the lamp door 14 Turn on the instrument and press the UTILITY key 15 Press the arrow keys to highlight Lamp Hours and press ENTER 16 Press ENTER to reset the lamp hours to zero ...

Page 124: ...22 Maintenance Replacing the Tungsten Lamp 116 GENESYS 10S Vis User Guide Thermo Scientific ...

Page 125: ...et Lets you calculate peak height from the tangential baseline in the graph Scanning Absorbance Enters the absorbance value Accept Name Accepts the displayed Name entry Test Name and Edit Units Add Character Adds a highlighted character to the Name entry Test Name and Edit Units Add nm Lets you add a wavelength and factor to the list in Multiwavelength tests and some Performance Verification tests...

Page 126: ...s Basic A T C and some Performance Verification tests Collect Baseline Starts the collection of the baseline Scanning only Concentration Sets the concentration value Conc of Standard Displays the entered concentration value Adv A T C Correction Mode Selects the mode for cell correction Discrete nms or Scan Cursor Goes to Cursor tracking mode to view data points in the graph Kinetics Scanning Curso...

Page 127: ...tests Diluent Volume Enters the volume of diluent added before measurement Dilution Multiplier in some Bio Tests Dilution Multiplier Displays the factor used to correct for sample dilution Display Activity Indicates whether results should include protein concentration DNA ε 260 Calculates the extinction coefficient DNA Factor Enter the factor to calculate DNA concentration DNA Bio Tests Edit Lets ...

Page 128: ...s WL2 x Factor Result Abs Ratio Abs Diff Multiwavelength tests Factor 3 31 Enters a factor to convert a datum to a result Abs WL3 31 x Factor Result Multiwavelength tests Graph Displays the graph of collected data Kinetics Scanning ID Enters the numeric identifier for measurement autoincrements during the test until turned off set to 0 Instrument Serial Number Displays the serial number of the ins...

Page 129: ...test from the Stored Tests Directory into active memory and sets the instrument to the test parameters Utility Lock Unlock Used to protect stored tests from accidental deletion or alteration asks for a password to allow the user to lock or unlock the file Utility Low High Limits Enters the lowest and highest acceptable results outside of which the result is flagged as Low or High Adv A T C Std Cur...

Page 130: ...lations in the graph Scanning Number of bases Enters the number of bases in the oligonucleotide Oligos tests Number of Matched Cuvettes Enters the number of cuvettes that will be run in the Correction Program maximum of 5 Number of Samples Enters the number of samples to be measured in the test Not available in Kinetics or Scanning Number of Standards Enters the number of standards to be measured ...

Page 131: ...nt Sample Volume Enters the total volume of sample under Dilution Multiplier in some Bio Tests Save Test Saves all parameters of the current test in internal memory for later recall all tests Scan speed Selects the speed nm min for a scan Slow Medium Fast Scanning tests only Screen Contrast Lets you improve visibility of the display by changing the contrast between the background and text Utility ...

Page 132: ...atistics OFF and or when instrument is OFF and or when test parameters are changed and or when test is saved or resaved in all test types except Kinetics Scanning Multiwavelength Std Concentration Enters the concentration of the analyte in the standard solution Stop wavelength Enters the ending wavelength for a scan Scanning tests only Stored Tests Directory Displays the list of tests stored in th...

Page 133: ...nits labels for results all stored tests except Abs Ratio Scanning Cell Growth Unselect Test Removes the tag of the highlighted test name to remove the test from the SmartStart menu Utility Stored Tests Directory Wavelength Enters values for the analytical wavelengths Parameter Description ...

Page 134: ...23 Parameters 126 GENESYS 10S Vis User Guide Thermo Scientific ...

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