PerkinElmer RamanMicro 200 Series, Getting Started Manual

Page: 83 / 98

Using the RamanMicro 200 Series . 83
11. Focus on the sample of polystyrene.
If you have a motorized stage you can use the joystick or Stage Position controls on the
SetupMicroscope XYZ Stage tab. Alternatively, click
Raman AutoFocus
to focus
using the strongest Raman signal.
If you do not have a motorized stage, you may find it useful to Monitor your sample to
view the Raman signal while manually adjusting the focus.
12. When the polystyrene sample is optimally focused for Raman signal, select the Setup
Instrument Advanced tab and click
Toolbox
.
The Adjustments Toolbox dialog is displayed.
13. Select .
The Frequency Calibration dialog is displayed.
14. Ensure that you have followed the instructions on the dialog, and then click
Next
.
The instrument will now perform the laser frequency calibration routine. This will take a
few minutes. When the calibration is complete, the result is displayed.
The instrument in now laser frequency calibrated.
In the unlikely event that the laser frequency calibration routine should fail, please perform a
wavelength calibration and repeat the laser frequency calibration.
NOTE: If the calibration fails again, please contact your PerkinElmer Service Representative.
SOP-13: Intensity Calibration
The intensity calibration must be performed using the National Institute of Standards and
Technology (NIST) Standard Reference Material. NIST SRM 2241 is the relative intensity
correction standard for Raman spectroscopy when using 785 nm excitation.
NOTE: NIST calibration is temperature dependent. If you intend to perform quantitative
analyses, you should ensure that your instrument is NIST calibrated at the
temperature at which data is to be acquired. Otherwise, any data should be used for
qualitative purposes only.
The NIST intensity correction is valid in the recommended operating temperature
range 20 ° C – 25 ° C. If you move the instrument to an environment outside this
temperature range, that is 15
°
C – 20
°
C or 25
°
C – 30
°
C, we strongly recommend
that you perform a NIST intensity correction at this new temperature. Contact your
PerkinElmer Raman Business Unit.