PerkinElmer RamanMicro 200 Series, Getting Started Manual

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30 . RamanMicro 200 Series Getting Started Guide
The change between the incident radiation and the Stokes/Anti-Stokes radiation represents a
change in the vibrational and/or rotational energy of the molecule. The frequency shifts from
the frequency of the incident beam are displayed on the x-axis of a Raman spectrum,
generally in relative wavenumbers (otherwise known as Raman Shift) and the intensity of the
bands are displayed in arbitrary units on the y-axis.
Figure 8 Raman spectrum of cyclohexane, showing Stokes, anti-Stokes and
Rayleigh scattering
Anti-Stokes x12
Rayleigh
Stokes
Raman Shift (cm
−1
)