PerkinElmer RamanMicro 200 Series, Getting Started Manual

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54 . RamanMicro 200 Series Getting Started Guide
16. Click
Next
.
The following dialog is displayed.
17. Use the arrows in the Align Video with Laser dialog to fine-tune the position of the
crosshairs to be in the center of the laser spot.
You will need to repeat the fine adjustment of the cross hairs using the
Align Video with
Laser
function for each microscope objective, but do not adjust the camera module.
Aligning the Raman Coupler
CAUTION
Adjustments to the Raman coupler can have a dramatic impact on the
performance of your Raman instrument. We recommend that you
contact your PerkinElmer Service Representative.
You should only adjust the Raman coupler if the signal throughput has decreased. This may
occur if you have moved the unit. The only adjustment to the Raman is the
xy
-adjuster at
the rear of the Raman module (see Figure 25). The procedure for alignment is as follows:
1. Place a flat sample of silicon on the sample stage.
We recommend that you purchase the sCAL Raman Calibration Standard (L1321843) for
this alignment procedure.
2. Loosen the 4 retaining screws at the coupler so that about 0.5 mm of thread is
showing.
3. Ensure that the Bright Field (BF) switch position is selected on the microscope, and that
the illumination is turned on.
4. In the Spectrum software, select
Raman Instrument
from the Setup menu.
The Setup Instrument tabs are displayed.
5. On the Setup Instrument Basic tab, ensure that
Microscope
or
Microscope XYZ
Stage
, as applicable, is selected as the Accessory.
6. Select the appropriate
Sample Holder
in the drop-down list.
7. Select the lowest magnification objective on the microscope.
8. Ensure that the correct objective it is selected in the Microscope Objective drop-down
list on the Setup Instrument Basic tab.
9. Select the Setup Microscope or Setup Microscope XYZ Stage tab, as applicable, and then
select
Live Video
.