PerkinElmer RamanMicro 200 Series, Getting Started Manual

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50 . RamanMicro 200 Series Getting Started Guide
Alignment of the Microscope
WARNING
Do not disconnect the microscope from the spectrometer. Only trained
PerkinElmer Service Representatives should attach and remove the fiber
optic cables that connect the two modules.
The RamanMicro 200 system consists of four primary components:
• The spectrometer module (containing the laser and the detector)
• The microscope
• The Raman module
•
The alignment camera module.
Please read and understand the general safety precautions before proceeding. If you do not
understand any of the details regarding general safety precautions, please contact
PerkinElmer before performing any of the following procedures.
Aligning the Microscope Objectives
NOTE: Do not touch the glass of the objective lenses when you select the appropriate
magnification. Do not push on an objective to rotate the nosepiece, but use the
revolving plastic ring.
The microscope objective nosepiece can fit up to 4 objectives.
1. Select the highest magnification objective lens on the microscope (either the 100x or
50x) by rotating the plastic ring on the objective nosepiece.
2. Select the Bright Field (BF) switch position on the microscope.
3. Place a slide with an easily recognizable feature on it (for example, a graticule slide).
4. In the Spectrum software, select
Raman Instrument
from the Setup menu.
The Setup Instrument tabs are displayed.
5. On the Setup Instrument Basic tab, ensure that
Microscope
or
Microscope XYZ
Stage
, as applicable, is selected as the Accessory.
6. Select the Setup Microscope or Setup Microscope XYZ Stage tab, as applicable, and then
select
Live Video
.
7. Move the stage until the feature is in focus and in the center of the crosshairs in the
Live Video.
Ensure that the image remains sharp and focussed.
8. Select the next highest magnification objective.