PerkinElmer RamanMicro 200 Series, Getting Started Manual

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Using the RamanMicro 200 Series . 73
17. If the microscope light-proof enclosure is fitted, close the doors.
18. Click .
The RamanMicro 200 will start collecting data in the Live display.
You can use Monitor to help you optimize the Exposure time and Laser power for your
sample. The data displayed on-screen when monitoring cannot be saved. To save data
you must collect spectra.
19. Adjust the
Laser power (%)
in the Live display to an appropriate value for your sample.
20. Enter an
Exposure time
(s).
Changing the exposure time will automatically acquire another background.
21. Click to exit the Live display.
You are ready to acquire data. For more information see SOP-6.
The most appropriate objective for your analyses will depend upon your sample. High-
magnification objective lenses give the most efficient Raman collection from thin samples,
such as films or wafers, but low-magnification objectives are better for macro samples such
as tablets, bulk plastics and tissue samples.
NOTE: Raman spectra are measured in the visible and near-infrared spectrum. Room
lighting and sunlight may contaminate your spectra. Take care not to misinterpret
room lighting or sunlight features as sample features.
SOP-6: Setting-Up and Saving Experimental
Parameters
This SOP is designed to act as a quick guide to help you start collecting data. The exact
setup for optimized spectral collection is sample dependent, and we recommend that you
modify these settings to suit your needs.
1. Start the Spectrum software and select
Raman
Instrument from the Setup menu.
The Setup Instrument tabs are displayed in the Dialog pane. They enable you to choose
the accessory, and setup and save the experimental parameters.
2. Select the Setup Instrument Basic tab.
Figure 39 Setup Instrument Basic tab