PerkinElmer RamanMicro 200 Series, Getting Started Manual

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82 . RamanMicro 200 Series Getting Started Guide
9. Ensure that you have followed the instructions on the dialog, and then click
Next
.
The instrument will now perform the wavelength calibration routine. This will take some
time. When the calibration is complete, the result is displayed.
10. Disconnect the neon calibration accessory and refit the screw-in cap to the port.
The instrument is now wavelength calibrated.
SOP-12: Laser Frequency Calibration
Laser frequency calibration should only be performed if the instrument has failed a
wavelength calibration verification.
To calibrate the microscope with the Microscope 50x objective selected:
1. Ensure that the RamanMicro 200 is switched on.
If not follow SOP-1.
2. Select the 50x objective lens on the microscope.
3. Place a polystyrene sample (L1181228) in a suitable sample holder.
4. Select
Raman Instrument
from the Setup menu.
The Setup Instrument tabs are displayed.
5. On the Setup Instrument Basic tab select
Microscope
or
Microscope XYZ Stage
as
appropriate from the
Accessory
drop-down list
6. Select
Microscope 50x
from the Objective drop-down list.
7. Select the appropriate
Sample Holder
in the drop-down list and load the sample holder
onto the sample stage, following the instructions on-screen if you have a motorized
stage.
8. Select the Dark Field (DF) switch position on the microscope and switch off the
illumination.
9. Cover the microscope with a dark cloth or, if fitted, close the light-proof enclosure doors
and ensure that caps are placed on any external fiber optic probes or accessories, to
minimize stray light from interfering with the calibration.
10. Select the Setup Instrument Advanced tab and ensure that the
Laser Power
is set to
100%.