PerkinElmer RamanMicro 200 Series, Getting Started Manual

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68 . RamanMicro 200 Series Getting Started Guide
17. Re-secure the hexagonal fixing screw to fix the height of the stage.
18. Select
Toolbox
and then select
Reset Stage
from the sub-menu.
19. Follow the prompts on-screen, as before.
You will need to repeat this process every time you adjust or install the stage.
CAUTION
Once you have reset the stage you should use the joystick ONLY to raise
or lower the stage. DO NOT try to adjust the stage manually, or you will
have to reset the stage.
When using the joystick, take care not to continue using the motors
when the stage has reached the lower or upper limits of travel. To see if
you have moved outside the limits, check the z-axis focus dials on the
right-hand side of the microscope. If the two wheels counter-rotate, you
will need to reset the stage. If you continue to move the stage, the
cable can become trapped and the motor could be damaged.
SOP-4: Sample Positioning using a Manual Stage
1. Lower the microscope sample stage.
2. Place the sample on a microscope slide and place it on the stage.
Figure 33 Positioning the sample under the objective lens
3. Select the 5x objective lens on the microscope.
NOTE: Do not touch the glass of the objective lenses when you select the appropriate
magnification. Do not push on an objective to rotate the nosepiece, but use the
revolving plastic ring.
4. Move the stage so that the sample is approximately lined up below the objective lens.
5. Select the Bright Field (BF) switch position on the microscope.
6. Switch on the illumination source.
The switch is located on the right side of the microscope frame.
7. Set the required light intensity using the adjustment knob on the right side of the
microscope frame base.
Place your
sample here