PerkinElmer RamanMicro 200 Series, Getting Started Manual

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Installing Your RamanMicro 200 Series . 55
10. Ensure that the sample is in focus.
11. Repeat steps 7–10 for each objective until the highest magnification is selected.
12. Switch to the Dark Field (DF) switch position on the microscope, and turn off the
illumination.
13. Cover the microscope with a dark cloth or, if fitted, close the light-proof enclosure doors
and ensure that caps are placed on any external fiber optic probes or accessories, to
minimize stray light from interfering with the calibration.
14. Click .
The RamanMicro 200 will start collecting data in the Live display.
15. Observe the data displayed in the Live display.
16. Very slowly move one of the coupler knobs clockwise and counter-clockwise until the
Raman signal is maximized.
Figure 25 Adjustment of the XY Raman coupler
17. Move the other knob in a similar manner until the Raman signal has maximized.
18. Return to the first knob and move again to maximize the Raman signal (the movement
should be much less than used the first time).
19. Return to second knob and do likewise.
20. Tighten each retaining screw a little and move the
xy
-coupler knobs to compensate
each time.
21. Tighten screws until the plate at the rear coupler is secure – do not over tighten.