PerkinElmer RamanMicro 200 Series, Getting Started Manual

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80 . RamanMicro 200 Series Getting Started Guide
4. Select
Raman Instrument
from the Setup menu.
The Setup Instrument tabs are displayed.
5. On the Setup Instrument Basic tab select
Microscope
or
Microscope XYZ Stage
as
appropriate from the
Accessory
drop-down list
6. Select
Microscope 50x
from the Objective drop-down list.
7. Select the appropriate
Sample Holder
in the drop-down list and load the sample holder
onto the sample stage, following the instructions on-screen if you have a motorized
stage.
8. Select the Setup Instrument Advanced tab and ensure that the
Laser Power
is set to
100%.
9. Select the Dark Field (DF) switch position on the microscope and switch off the
illumination.
10. Cover the microscope with a dark cloth or, if fitted, close the light-proof enclosure doors
and ensure that caps are placed on any external fiber optic probes or accessories, to
minimize stray light from interfering with the calibration verification.
11. Focus on the polystyrene sample.
If you have a motorized stage you can use the joystick or Stage Position controls on the
SetupMicroscope XYZ Stage tab. Alternatively, click
Raman AutoFocus
to focus
using the strongest Raman signal. The Focus Limits should be set to
Full Range
.
If you do not have a motorized stage, you may find it useful to Monitor your sample to
view the Raman signal while manually adjusting the focus.
12. When the polystyrene sample is optimally focused for Raman signal, select
Toolbox
on
the Setup Instrument Advanced tab.
The Adjustments Toolbox dialog is displayed.
13. Select .
The Wavelength Calibration Verification dialog is displayed.
14. Ensure that you have followed the instructions on the dialog, and then click
Next
.
The wavelength calibration verification routine begins. This will take a few minutes.
When the verification is complete, the result is displayed.
15. Remove the polystyrene sample and store safely.
The instrument is now ready for use.