PerkinElmer RamanMicro 200 Series, Getting Started Manual

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Using the RamanMicro 200 Series . 67
9. Re-secure the fixing screw to fix the height of the stage.
10. Select the Setup Microscope XYZ tab.
Figure 32 Setup Microscope XYZ Stage tab showing the Live Video view
11. Select
Toolbox
and then select
Reset Stage
from the sub-menu.
12. Follow the prompts on-screen.
During the Reset Stage procedure, you will be prompted to use the joystick to move the
stage to the point of lowest travel. If you place your hand carefully on the coarse
adjustment control, you will feel it move (as it starts to counter-rotate with the fine
focus control) when you have reached the lower limit. Stop using the joystick at this
point.
13. When the stage has been reset, use the joystick or the stage position controls in the
software to move the microscope stage up to 0 mm (approximately 25 mm travel).
The stage will stop at some point between 0 mm and 1 mm, at the maximum length of
travel for the z-axis motor.
14. Ensure that you are now at the maximum point of travel for the stage by turning the
coarse adjustment control.
It may move slightly, but should be nearly at the maximum.
15. Place your sample on the stage.
Using a hexagonal key, loosen the fixing screw on the side of the microscope
(Figure 31).
16. Carefully, move the stage up manually until it is at the highest position possible without
the sample touching the objective.
CAUTION
Be very careful not to touch the objective.
The performance of the Raman microscope will be affected by dirt on
the surface of, or damage to, the objectives.
NOTE: When using long working-distance objectives (for example, Long Working Distance
Objective Lens Kit, L1323347), the working distance depends on the objective
(usually, 2–10 mm).