PerkinElmer RamanMicro 200 Series, Getting Started Manual

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72 . RamanMicro 200 Series Getting Started Guide
10. Select the Setup Microscope XYZ tab, and then select the Live Video view.
Figure 38 Setup Microscope XYZ Stage tab showing the Live Video view
11. Use the joystick, or click Stage Position and then use the controls, to move the stage
so that the sample is approximately lined up below the objective lens and then focus on
the sample.
The crosshairs indicate the spot where analysis takes place.
The joystick operates in the following manner:
• Left / Right joystick movement gives x movement of the stage.
•
Top / Bottom joystick movement gives y movement of the stage.
•
Twisting the joystick gives z movement of the stage (if purchased):
Left twist (counter clockwise) gives upward stage movement;
Right twist (clockwise) gives downward stage movement.
NOTE: You can also click
Video Autofocus
to perform an automatic video focus when
you are close to the sample surface (when the microscope illumination is on and the
switch position set to Bright Field). Alternatively, select the Dark Field switch position
on the microscope, and click
Raman AutoFocus
to focus using the strongest
Raman signal.
12. If higher magnification is required, select the next most powerful objective lens on the
microscope.
The microscope is supplied with 5x, 20x and 50x objective lenses.
NOTE: Do not touch the glass of the objective lenses when you select the appropriate
magnification. Do not push on an objective to rotate the nosepiece, but use the
revolving plastic ring.
13. Select the appropriate objective lens from the
Microscope Objective
drop-down list.
14. Focus on the sample and, if necessary, move the stage in
x
and
y
directions to
accurately position the sample.
15. When the sample is correctly positioned, switch off the illumination source.
16. Select the Dark Field (DF) switch position on the microscope.