PerkinElmer RamanMicro 200 Series, Getting Started Manual

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Using the RamanMicro 200 Series . 77
5. Ensure that laser safety precautions are being observed, and then click Continue.
If you have any doubts about the required safety precautions click Cancel.
When the laser is on and the shutter is open the Laser Status icon on the Measurement
bar will be updated to Class 3B Laser Status . Laser light will be emitted from the
probe during data collection and when the software is Monitoring.
The probe can now be positioned so as to achieve the best spectrum. See SOP-8.
SOP-8: Sample Positioning when using a Fiber Optic
Probe
Monitor or Preview modes allow you to collect spectral data while optimizing the probe
position and the laser power and exposure time, for example, to enhance the signal-to-noise
ratio of spectral data. When measuring samples inside a container (for example, a bottle) it
can be used to ensure that you are focused on the material of interest and not the container.
The data that is displayed on the Live tab when monitoring cannot be saved.
NOTE: To see the Live display, the
Show live display
option must be selected on the Setup
Instrument Data Collection tab.
1. Click .
The Live tab will be displayed and real-time spectral acquisition will commence.
2. Adjust the
Laser power (%)
on the Live tab to an appropriate value for your sample.
3. Adjust the Exposure time (s).
This will automatically acquire another background.
The sample may now be positioned and aligned correctly for optimized data collection.
The spectral data can be optimized by adjusting the distance between the fiber optic
probe and the sample.
NOTE:
When positioning your sample, you should ensure that you are not collecting data
from any substrate used to support your sample. It is advisable to have 1–2 cm of
free space behind the sample. Alternatively, you can subtract the spectrum of your
substrate from your sample spectrum.
4. Click to exit the Live display.
You can now collect and save spectral data. To set up an experiment and collect data using
the FOP please refer to SOP-6
.
NOTE: If you have
Show Live Display
selected on the Setup Instrument Data Collection tab
and you select
Preview
on the Sample Table, then you can preview each sample in
the Live display after pressing
Scan
. When you have adjusted the settings, click
Scan
again to collect data.