PerkinElmer RamanMicro 200 Series, Getting Started Manual

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Using the RamanMicro 200 Series . 79
Figure 41 System verification / calibration flow diagram
If wavelength calibration verification fails and the Description of the calibration report
spectrum states that peak wavelengths are outside limits, then wavelength and laser
frequency calibrations should be performed. When these calibrations have been completed
successfully, repeat the wavelength verification to ensure that the instrument has been
brought back within specification.
If the wavelength calibration verification fails because the spectral intensity is low, then this
may also be due to the calibration sample holder not being placed in the sample accessory,
or that it is not correctly positioned, optimally focused or because the laser key is not
switched on, or the laser power is too low. Then repeat the wavelength calibration
verification to ensure that instrument has been brought back within specification. If the
instrument fails the wavelength calibration verification again, please contact PerkinElmer.
NOTE: Stray light could cause the wavelength calibration verification to fail. If a test fails,
particularly if you are using the microscope without an enclosure, ensure that no light
is entering the Raman system.
SOP-10: Wavelength Calibration Verification
To perform the Wavelength Calibration Verification with the Microscope 50x objective
selected:
1. Ensure that the RamanMicro 200 is switched on.
If not follow SOP-1.
2. Select the 50x objective lens on the microscope.
3. Place a polystyrene sample (L1181228) in a suitable sample holder.
Wavelength Calibration
Verification
Laser Frequency
Calibration
Wavelength
Calibration
Finished.
Instrument ready
for use
Check Laser Key is
turned on and laser
power is set to
100%
Check position and
focus of polystyrene
sample
Completed
Fail
Peak
wavelengths
outside limits
Fail
Intensity
low
Pass