Nikon Eclipse Ti Series, Setup Manual

Page: 288 / 329

Chapter 4 Setting Up the Microscope
4.2 Setup Procedure
2-30
1
TIRF-PAU illuminator installation
1. Remove the clamping screws 1 and 2 from the part used in PAU illumination operation.
2. Press the part used in PAU illumination operation into the microscope to secure it.
3. Attach the two support legs (for two areas) on the part used in PAU illumination operation to
adjust the height.
4. Attach both parts used in TIRF-microscopy operation and for PAU illumination operation to
secure them. (Remove the clamping screw 1 in advance, or you may fail to attach the parts.)
5. Attach both the optical fibers for the parts used in TIRF-microscopy operation and PAU
illumination operation. Adjust the height of each part to approx. 110 mm as shown in the figure
to secure it.
6. Perform optical adjustments for the parts used in TIRF-microscopy operation and PAU
illumination operation.
This is the end of the TIRF-PAU illuminator installation.
LASE
R RADIA
TIO
N
AV
OID
E XPO
SU
RE T
O BEA
M
CLA
SS
3B LA
SE
R PR
ODU
CT
AV
OID
EX
POSUR
E
LA
SE R
LIG
HT
IS E
MIT
TE
D
FR OM
O
BJ
EC TIV
E
AP
ER TU
RE
CAUTION – CLA
SS 3B
LASER RADIAT
ION WHEN OPE
N
AVOID EXPOSU
RE TO THE BEA
M
VORSICHT – LA
SERSTRAHLUN
G
KLASSE 3B,
WENN ABDECK
UNG GEÖFFN
ET
NICHT DEM STR
AHL AUSSETZE
N
AVOID EXPOSU
RE LASER LIGHT
IS EMITTED FROM THIS A
PERTURE
CAUTION – CLA
SS 3B LASER RADIAT
ION WHEN OP
EN AVOID EXPOSU
RE TO THE BE
AM VORSICHT – LA
SERSTRAHLUN
G
KLASSE 3B,
WENN ABDEC KUNG GEÖFF
NET NICHT DEM ST
RAHL AUSSET
ZEN
CAUTION – CLA
SS 3B LASER RADIAT
ION WHEN OP
EN
AVOID EXPOS
URE TO THE B
EAM
VORSICHT – LA
SERSTRAHLUN
G
KLASSE 3B,
WENN ABDEC
KUNG GEÖFF
NET
NICHT DEM ST
RAHL AUSSET
ZEN
Figure 4.2-1
Optical fiber for TIRF
TIRF illuminator unit
Fixing screw 1
PAU illuminator unit
Support legs
Fiber for PAU
Fixing screw 2
Approx.
110 mm