NEW BRUNSWICK SCIENTIFIC BioFlo CelliGen 115, Manual To Operations

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BioFlo/CelliGen 115
•
M1369-0050 User’s Guide
7.3 D
ISSOLVED
O
XYGEN
(DO) P
ROBE
P
REPARATION
..................................................... 66
7.3.1 Inspecting the DO Probe.................................................................................. 66
7.3.2 DO Probe Preparation..................................................................................... 66
7.3.3 DO Probe Installation ...................................................................................... 67
7.3.4 DO Probe Polarization .................................................................................... 68
7.3.5 DO Probe Calibration: Setting Zero............................................................... 68
7.3.6 DO Probe Calibration: Setting Span .............................................................. 70
7.4 L
EVEL
P
ROBE
C
ALIBRATION
..................................................................................... 70
7.5 A
BOUT
P
UMP
C
ALIBRATION
...................................................................................... 71
8 VESSEL STERILIZATION.......................................................................................... 72
8.1 I
NITIAL
P
REPARATION FOR
A
UTOCLAVING
................................................................ 73
8.2 A
UTOCLAVING THE
V
ESSEL
...................................................................................... 74
8.2.1 Sterilization Time and Temperature................................................................. 75
9 REINSTALLING THE VESSEL ASSEMBLY .......................................................... 76
9.1 R
EINSTALL THE
V
ESSEL
A
SSEMBLY
.......................................................................... 76
9.2 L
OAD
P
UMP
T
UBING
................................................................................................. 76
9.3 C
ONFIRM P
H C
ALIBRATION
....................................................................................... 78
9.4 I
NSTALL
L
IQUID
A
DDITION
S
YSTEMS
........................................................................ 78
9.4.1 Addition Tubing Size ........................................................................................ 79
9.5 R
ECONNECT
G
ASES
................................................................................................... 80
9.6 I
NSTALL
T
EMPERATURE
(RTD) P
ROBE
..................................................................... 80
10 CASCADE CONTROL ............................................................................................. 81
10.1 C
REATING A
C
ASCADE
.............................................................................................. 82
11 ABOUT PUMPS ......................................................................................................... 84
11.1 P
UMP
A
SSIGNMENT
................................................................................................... 84
11.2 P
UMP
S
ETPOINT
......................................................................................................... 85
11.3 P
UMP
C
ONTROL
M
ODE
.............................................................................................. 87
11.4 P
UMP
F
LOW
R
ATE
& C
ALIBRATION
M
ETHODS
......................................................... 87
11.5 P
UMP
P
ERIOD
............................................................................................................ 88
11.6 U
SING
L
EVEL
P
ROBES TO
P
ROGRAM
F
EED
P
UMPS
.................................................... 89
11.6.1 Setting a Feed Pump to Add Liquid ................................................................. 89
11.6.2 Setting a Feed Pump to Harvest....................................................................... 90
11.6.3 Level Control Off.............................................................................................. 90
11.6.4 Pump Calibration ............................................................................................. 90
12 USING THE SETUP SCREEN................................................................................. 92
12.1 C
ONTROLLER
S
ETUP
................................................................................................. 92
12.1.1 Gas Control ...................................................................................................... 94
12.2 S
YSTEM
S
ETTINGS
..................................................................................................... 97
12.2.1 Resetting Date/Time ......................................................................................... 97
12.2.2 Updating Software............................................................................................ 97
12.3 H
ARDWARE
S
ETUP
.................................................................................................... 98
12.3.1 Identifying Utility Station(s) Added................................................................ 100