5
New Brunswick Scientific
User’s Guide
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3.1 System
BioFlo/CelliGen 115 is a versatile fermentor/bioreactor that provides a fully equipped
system in one compact package. It can be employed for batch, fed batch or continuous
culture with process control for pH, dissolved oxygen (DO), agitation, temperature,
pump feed, antifoam and foam/level.
Systems can be configured as either control stations or utility stations. Each individual
stand-alone system is a control station. One control station can run up to two
additional utility stations, which are dependent on the control station.
3.2 Vessels
One of the most versatile features of the BioFlo/CelliGen 115 is the wide variety of
glass vessels available. There are two types of vessels, non-jacketed (heat-blanketed)
and water-jacketed. Each type of vessel is available in four sizes: 1.3 liters, 3.0 liters,
7.5 liters and 14.0 liters. Ports in the headplate are provided for, but not limited to, the
following purposes: inoculation; base and acid addition; a thermowell for a resistance
temperature detector (RTD); a foam probe; a sparger; a harvest tube; a sampling tube; an
exhaust condenser; and dissolved oxygen (DO) and pH electrodes. The drive bearing
housing is also located on the headplate
(see Figures 3 & 8)
.
3.3 Agitation
System
A removable agitation motor located on top of the bearing housing on the headplate is
connected to the agitation shaft with a direct drive coupling or a magnetic coupling.
It can be easily disconnected before autoclaving the vessel and easily replaced after
sterilization. The motor will provide a speed range from 50 to 1200 RPM for
fermentation with direct drive, from 25 to 400 RPM for cell culture with direct drive,
or from 25 to 200 RPM for cell culture with magnetic drive. The process control
software ensures agitation speed control throughout the speed range.
It is possible to cascade Dissolved Oxygen (DO) to Agitation (AGIT) so the agitation
speed will vary between the user-specified minimum and maximum setpoints in order
to maintain the set percentage of DO.
(See Section 10 for further information on
setting up cascades.)
Default P & I (proportional & integral) values are preset at the factory.
We strongly
recommend that you maintain the factory-set parameters.
