6
BioFlo/CelliGen 115
y
M1369-0050
User’s Guide
3.4 Temperature
Control
The culture temperature setpoint may be selected within the range from 20
°
C above
coolant temperature to 70
°
C for 1.3- to 7.5-liter vessels, and from 20
°
C above coolant
temperature to 65
°
C for 14.0-liter vessels. It is controlled by the process control
software which then sends information to either a heater blanket and cooling coil or to
a water jacket. The media temperature is sensed by a Resistance Temperature
Detector (RTD) submerged in the thermowell.
Default P & I (proportional & integral) values are preset at the factory.
We strongly
recommend that you maintain the factory-set parameters.
3.5 Aeration
Up to four gases, including air, nitrogen, carbon dioxide and oxygen, can be
introduced into the media through the ring sparger or optional microsparger. The flow
rate is controlled manually by one, two, three or four rotameter(s) or automatically by
thermal mass flow controller (TMFC), according to the definition of your system. The
TMFC is regulated automatically according to values set via the control station
touchscreen.
The gas mix can either be controlled manually by adjusting the flow of gases through
their rotameters or automatically if 4-gas mixing was purchased as an option.
(For
further information on cascading, see Section 10.)
4-gas mixing allows the system to
automatically calculate the gas mix in response to culture needs.
Default P & I (proportional & integral) values are preset at the factory.
We strongly
recommend that you maintain the factory-set parameters.
3.6 pH
Control
pH is controlled in the range of 2.00-14.00. The pH is sensed by a gel-filled pH probe
(see Figures 11 & 28)
. Control is maintained by a P & I (proportional & integral)
controller which operates peristaltic pumps, assigned to perform acid or base addition,
or which controls the use of gas(es) for this purpose. The user can also select a
deadband value to control pH within the user-assigned range: no acid or base will be
added when the pH value falls within the deadband tolerance above or below the
setpoint.
Default P & I (proportional & integral) values are preset at the factory.
We strongly
recommend that you maintain the factory-set parameters.
