manualshive.com logo in svg

NEW BRUNSWICK SCIENTIFIC BioFlo CelliGen 115, Manual To Operations

Share
Download
NEW BRUNSWICK SCIENTIFIC BioFlo CelliGen 115 Manual To Operations Download Page 125

   113 

New Brunswick Scientific 

 

User’s Guide 

16.6 Replacement 

Parts 

 

The following lists of replacement parts are provided for your convenience.  Using  
the NBS part number will facilitate processing of your order by your local NBS 
distributor.  For a complete list of spare parts, please visit the NBS website 
(www.nbsc.com) or contact your local sales representative. 
 

Heaters & Heater Blankets 

1.3L, 3.0L 

M1369-3107 

Water Jacket Heaters 

7.5L, 14.0L 

M1369-3108 

1.3L M1369-8021 
3.0L M1369-8022 
7.5 M1369-8020 

Heat Blankets 

14.0L M1369-8023 

Motors 

Direct Drive Cell Culture 

All sizes 

M1369-3135 

1.3L, 3.0L 

M1369-3120 

Direct Drive Fermentation 

7.5L, 14.0L 

M1369-3125 

Magnetic Drive  

All Sizes 

M1369-3130 

Impellers 

6-Blade Rushton type 52mm 

1.3L, 3.0L 

M1273-9291 

6-Blade Rushton type 59mm 

7.5L 

M1273-9292 

6-Blade Rushton type 74mm 

14.0L 

M1273-9293 

1.3L, 3.0L 

M1273-9206 

Pitched Blade 

7.5l, 14.0L 

M1273-9207 

 

   

Summary of Contents for BioFlo CelliGen 115

Page 1: ...PO Box 4005 44 Talmadge Rd Edison 08818 4005 USA 1 800 631 5417 1 732 287 1200 bioinfo nbsc com www nbsc com Guide to Operations BioFlo CelliGen 115 Benchtop Fermentor Bioreactor MANUAL No M1369 0050...

Page 2: ...ii BioFlo CelliGen 115 M1369 0050 User s Guide...

Page 3: ...oad Shanghai 200235 P R China Tel 86 21 6484 5955 or 5966 Fax 86 21 6484 5933 E mail nbschc online sh cn THE NETHERLANDS New Brunswick Scientific BV Kerkenbos 1101 6546 BC Nijmegen P O Box 6826 6503 G...

Page 4: ...rated as described in this manual If operational guidelines are not followed equipment damage and personal injury can occur Please read the entire User s Guide before attempting to use this unit Do no...

Page 5: ...Manual Conventions NOTE Notes contain essential information that deserves special attention CAUTION Caution messages appear before procedures which if caution is not observed could result in damage to...

Page 6: ...bly and our obligation under this warranty is limited to repairing or replacing the instrument or part thereof which shall within 1 year after date of shipment prove to be defective after our examinat...

Page 7: ...________________________ SERIAL NUMBER ________________________________ The above information can be found on the electrical specification plate Purchased with the following installed options ________...

Page 8: ...FTWARE 8 4 INSTALLATION 9 4 1 PHYSICAL LOCATION 9 4 2 ENVIRONMENT 9 4 3 INSTALLING THE CONTROL CABINET 10 4 4 CONNECTING UTILITY CABINETS 12 4 5 UTILITIES 14 4 5 1 Electrical Requirements 14 4 5 2 Wat...

Page 9: ...e 42 4 8 22 Install Headplate 42 4 8 23 Install Vessel 43 4 8 24 Install Motor Assembly 43 4 8 25 Make All Connections 43 4 9 MAIN POWER SWITCH 44 4 10 OPTIONAL BIOCOMMAND SOFTWARE 45 5 SPECIFICATIONS...

Page 10: ...UMP TUBING 76 9 3 CONFIRM PH CALIBRATION 78 9 4 INSTALL LIQUID ADDITION SYSTEMS 78 9 4 1 Addition Tubing Size 79 9 5 RECONNECT GASES 80 9 6 INSTALL TEMPERATURE RTD PROBE 80 10 CASCADE CONTROL 81 10 1...

Page 11: ...5 13 11 HARVESTING PROCEDURE 106 13 12 SHUTDOWN PROCEDURE 106 14 ESSENTIAL OPERATING TIPS 108 14 1 PRECAUTIONS FOR GLASS VESSEL ASSEMBLY 108 14 2 EXHAUST CONDENSER EXHAUST FILTERS 108 14 3 INSTALL A D...

Page 12: ...3 21 APPENDIX C FERMENTATION TECHNIQUES 125 21 1 MEDIA FORMULATION 125 21 2 ANTIFOAM FORMULATION 126 21 3 TUBING SIZE 126 21 4 ACID BASE 127 21 5 GLUCOSE FEED 127 21 6 RECOMMENDED PROCESS CONTROL SETT...

Page 13: ...can build up possible shattering the vessel and endangering personnel As soon as you open the airflow valve s verify by feel that air is flowing freely from the exhaust If not immediately close the v...

Page 14: ...hat the thermowell does not touch the cooling coil 32 Do not install the pH port adaptor in the headplate before inserting the probe 34 64 Do not install the dO2 port adaptor in the headplate before i...

Page 15: ...clave pressure only when the temperature has dropped below 90 C Use slow exhaust 75 Proper pH control is critically dependent on tubing size which should be as small as possible 79 Never clean the ves...

Page 16: ...NBS Sales Order Department 2 3 Basic Components You should have at least the following components which will be described in greater detail later in this manual Control Cabinet with Touchscreen Beari...

Page 17: ...d addition a thermowell for a resistance temperature detector RTD a foam probe a sparger a harvest tube a sampling tube an exhaust condenser and dissolved oxygen DO and pH electrodes The drive bearing...

Page 18: ...em The TMFC is regulated automatically according to values set via the control station touchscreen The gas mix can either be controlled manually by adjusting the flow of gases through their rotameters...

Page 19: ...nitored during batch fermentation by a foam level probe located in the headplate The controller operates the antifoam assigned pump that adds chemical defoamer into the vessel as needed The internal l...

Page 20: ...n only User s kits and start up kits are available from NBS with many of the commonly required items including a selection of tubing clamps filters connectors and addition vessels Speak to your NBS sa...

Page 21: ...plus vessel contents and any applicable ancilliary equipment Also ensure that there is enough space around the back and the front of the BioFlo CelliGen 115 for proper operation and access Allow at l...

Page 22: ...it is completely assembled and all connections have been made you will plug the power cord into a suitable electrical outlet Figure 1b Front View NOTE Figures 1a 1b 1c 1d represent one possible contro...

Page 23: ...Utility stations do not have a fan Service Connections see Figure 1d Gas Connections see Section 4 5 3 Label with electrical specifications unit serial number Cabinet Input port see Section 4 4 Cabin...

Page 24: ...lectrical specification plate located on the rear panel of the cabinet and the control schematics supplied with the unit 4 4 Connecting Utility Cabinets If you have a control station and one or two ut...

Page 25: ...he control station s output COM port and to the utility station s input COM port as shown in Figure 1e Verify that the cable is securely connected to both cabinets Figure 1e Connecting Cabinets To add...

Page 26: ...ons 4 5 1 4 5 3 Table 1 Service Connections Service Utility Requirement Connection 120 VAC 50 60 Hz Single Phase 10 Amp fluctuations not to exceed 10 120 VAC 1ph field wired to 15 Amp disconnect in pa...

Page 27: ...lied with an open end for water in and the drain and with quick connect fittings to attach to the cabinet The tubing NBS part number P0740 1631 has an inner diameter of inch and an outer diameter of 7...

Page 28: ...emically inert tubing such as Marprene Pharmed etc may be used as well Gas inlets plugged with black plastic are unavailable to your configuration and must remain plugged WARNING Do not use this equip...

Page 29: ...it contains essential information cautions and warnings to protect your safety and the safety of your equipment Connect the barbed sparge connector here If the controller is equipped with an automatic...

Page 30: ...e familiarize yourself with these cautions Never allow hot glass to touch cold water or a cold surface Never rest the vessel on an uneven surface Never drag or roll the vessel across any surface Avoid...

Page 31: ...ts the impeller shaft at risk for damage Figure 2a WRONG Handling of Drive Assembly The correct way which is resting the drive assembly and headplate on the surface see Figure 2b below protects the im...

Page 32: ...l views in Figure 3 on the following page has two large viewing windows so the culture remains visible for inspection WARNING NEVER cut any portion of the heat blanket NEVER fold the heat blanket or p...

Page 33: ...following diagrams before proceeding with the vessel assembly You may find it more practical to change the arrangement the variety of ports and adapters will easily accommodate your needs HEAT BLANKE...

Page 34: ...te Figure 4 1 3L Headplate NOTE The RTD thermowell port should only be used for its intended purpose SPARGER COOLING COIL RTD THERMOWELL see NOTE below Each bolt is a possible mounting position for a...

Page 35: ...3 0L Headplate NOTE The RTD thermowell port should only be used for its intended purpose Each bolt is a possible mounting position for a bottle holder dO2 PROBE Mounting position for Sampler Assembly...

Page 36: ...round the vessel taking care to leave one of the viewing windows facing forward You will probably want to orient the blanket so the power cord connection is out of the way 2 Secure the blanket by over...

Page 37: ...U shaped rubber bumper equidistantly around the inside of the clamping ring there are three pieces for 1 3L 3 0L vessels and two larger pieces for 7 5L and 14 0L vessels Press each section securely a...

Page 38: ...t At the bottom is a metal base plate to provide additional security against breakage In operation the jacked vessel sits on the Jacket Water Heater The jacket water heater is designed so that the ves...

Page 39: ...IFTING HANDLE BEARING HOUSING HEADPLATE TOP CLAMPING RING BOTTOM CLAMPING RING BASE PLATE SPARGER COOLING WATER OUTLET TUBE THERMOWELL BAFFLE WATER JACKET COOLING WATER OUTLET COOL LOOP RETURN tubing...

Page 40: ...e ring half 3 Bring the two halves of the headplate clamping ring together under the vessel flange with the mounting plates on the bottom for easy access from below 4 Align the mounting plates with th...

Page 41: ...e solenoid valve cable and the RTD cable are plugged into the Power Controller 2 Set the temperature control mode to Off 3 Check that the temperature reading is higher than 5 C 4 Allow water to enter...

Page 42: ...ide one impeller onto the agitation drive shaft from the bearing housing Position this lower impeller according to the table below Clamp it down in place Then install the second upper impeller in the...

Page 43: ...t screw that is easiest to access You only need to adjust one 3 0L 7 5L 14 0L Vessel Cooling Coil 1 From beneath the headplate insert both ends of the coil into the Cooling Coil In port and the Coolin...

Page 44: ...ampler tube in the sample port If you are using the 1 3L vessel the sampler tube and harvest tube are welded into the same tri port to save space 2 Finger tighten the knurled adapter on the sampler tu...

Page 45: ...one at a time 1 Working from above the headplate insert the level probe into the appropriate port 2 Finger tighten the knurled adapter 4 8 14 Install Addition Tube s Insert addition tubes and or tri...

Page 46: ...e the top portion of the knurled port adapter part of the probe kit onto the probe 4 Slide the two white ferrules onto the probe the narrower one on top of the deeper cup shaped one 5 Gently slide the...

Page 47: ...d adapter assembly 4 8 16 Install dO2 Probe NOTE Prior to installation any dissolved oxygen probe you are using should be inspected for damage and replaced if necessary NOTE To avoid damage to the pro...

Page 48: ...lide the white Teflon O ring onto the probe then follow with the black 12mm port adapter O ring 7 Do not yet close up all the elements of the port adapter 8 Gently insert the probe into the appropriat...

Page 49: ...s secure 5 Attach the exhaust filter respecting the direction of flow if stamped on the filter to the condenser outlet Secure the filter with a plastic tie 6 Connect silicone tubing to the inlet port...

Page 50: ...on is easily autoclaved in place on the vessel If you are using the sampler install the kit as follows using Figures 14a 14b below for reference 1 Remove a headplate clamping nut adjacent to the locat...

Page 51: ...SAMPLER TUBE SYRINGE SYRINGE FILTER HEADPLATE SAMPLER BOTTLE HOLDER SAMPLER BOTTLE HEADPLATE CLAMPING NUT HEADPLATE CLAMPING SCREW SYRINGE THUMB CLAMP 1 8 SILICONE TUBING REDUCING ELBOW to 3 16 tubin...

Page 52: ...it will go into the open end of the filter Although the syringe will lodge there and hang freely in place you can add a plastic tie for security 9 Close the plunger 10 Remove the cap from one of the...

Page 53: ...th a plastic tie to the foam exhaust tube in the headplate 8 After autoclaving you will position the bottle holder where you want it then finger tighten the clamping nut Figure 15 Foam Trap 4 8 20 Plu...

Page 54: ...You may find it convenient to squeeze the tab with your thumb 2 Carefully lower the headplate easing all of its attachments into the vessel without hitting the glass or the baffle inside if this is a...

Page 55: ...nectors Be sure to keep the water line quick connects to the left Figure 16 Vessel Location 4 8 24 Install Motor Assembly 1 Position the motor assembly on top of the bearing housing using the locating...

Page 56: ...already done so connect the sparge line silicone tubing to the inlet filter WARNING Never block the exhaust to pressurize the vessel see Section 4 6 4 9 Main Power Switch The main power switch is loc...

Page 57: ...nected to the control cabinet and plugged into a suitable power outlet 4 10 Optional BioCommand Software If you are using NBS supervisory software be sure to consult your BioCommand user s manual for...

Page 58: ...e to read and log the current values of any parameters temp pH DO air flow pump flow rate levels and agitation that are monitored The data can also be stored plotted and afterwards transferred to othe...

Page 59: ...ng blanket and immersed stainless steel coiling coil Water jacketed Vessels Water jacket heater and circulation loop Temperature Sensor Platinum RTD probe Pt 100 Drive Magnetic Drive or Direct Drive I...

Page 60: ...xhaust Condenser w o Exhaust Condenser Width Depth Height Width Height Water Jacketed in cm in cm in cm in cm in cm 1 3L Vessel 11 5 29 11 5 29 20 5 52 9 3 24 16 41 3 0L Vessel 11 5 29 11 5 29 22 5 56...

Page 61: ...49 New Brunswick Scientific User s Guide...

Page 62: ...ructions to touch and hold the target each time it appears Usually you will be prompted to touch the four corners of the screen twice in succession NOTE For optimal results be sure to stand or sit in...

Page 63: ...115 controller can run as many as three stations the dark blue Unit Tab identifies which vessel s operating parameters are being displayed in the sample screen the unit being displayed is labeled Uni...

Page 64: ...on your system s configuration This one is equipped with Gas Mix OPERATING MODE SCREEN ACCESS buttons UNIT TAB NOTE The dark blue button usually represents the screen being displayed Current DATE TIME...

Page 65: ...oop Out The current percent output for each loop This is an automatic control function to maintain current readings within the setpoint tolerance range Control Mode Depending on the loop the control m...

Page 66: ...ou access to the Pump Gauges screen where the three pump gauges are displayed providing both current readings and the opportunity to change pump settings Setup This screen allows you to make changes t...

Page 67: ...typing the entry pressing the OK key saves the entry and closes the keypad LoopName Units The action of this loop Agitation is measured in RPMs Decimal Places Press the appropriate button to display v...

Page 68: ...e Section 11 3 for details To change operating modes for any of the displayed loops 1 Press either the LoopName or the Control Mode box in the row for the appropriate loop to open the loop s GAUGE scr...

Page 69: ...red setpoint Use the white CLEAR button at any time before Step 5 to empty the setpoint edit box 5 Press OK to save the setpoint and return to the GAUGE screen or CANCEL to return without saving the s...

Page 70: ...ation Screen 6 2 10 Cascade Screen A cascade is a control function that uses the output of one loop to influence the action and output of one or more other loop s This screen see Figure 24 allows the...

Page 71: ...Section 11 1 Figure 25 Pump Screen 6 2 12 Setup Screen This master screen is actually comprised of three screens see Figures 26 26a 27 accessed by tabs which are used to set up the controller system s...

Page 72: ...ftware Firmware version installed and to update Software via the USB port see Section 12 2 2 TAB The UNIT NAME is user selected Press this box then use the pop up keypad to type in the name The VESSEL...

Page 73: ...igure 27 Hardware Setup Screen Use this screen to view and add hardware for as many as 3 units installed in the system and to set Unit IDs for software see Section 12 3 Use this pane to choose softwar...

Page 74: ...the pH probe before autoclaving it with the vessel 1 If you have not already done so connect the pH probe to the pH connector on the control cabinet see Figure 1d using the appropriate cable 2 Turn th...

Page 75: ...a few times using the same two buffer solutions NOTE The pH calibration should be checked after autoclaving immediately prior to inoculation Take a sample from the vessel and compare the pH value dis...

Page 76: ...before inserting the probe Follow the steps below to fit the pH port adapter onto the probe first then insert the probe and adapter into the headplate Figure 28 pH Probe with Port Adapter exploded Wit...

Page 77: ...r assembly CAUTION We recommend that you avoid the use of hydrochloric acid HCl with the BioFlo CelliGen 115 for pH control or any other purpose because HCl corrodes stainless steel Over time it will...

Page 78: ...de fermentation and cell culture It is strongly recommended that you maintain the factory set parameters Nevertheless P I gains for the DO loop can be modified by the operator using the touchpad on th...

Page 79: ...elf in case of accidental breakage 2 Lightly coat the dO2 probe with glycerol CAUTION Do not install the dO2 port adaptor in the headplate before inserting the probe Follow the steps below to fit the...

Page 80: ...er into the port 10 Adjust the probe to the desired height then nesting the ferrules close the top portion of the adapter onto the bottom portion 11 Finger tighten the knurled adapter assembly 7 3 4 D...

Page 81: ...not be present In this case manually turn the N2 loop on from the SUMMARY screen or manually turn on the rotameter and set it to 1 20 SLPM depending on vessel size and flow controller 4 In approximate...

Page 82: ...s see Figure 30a If the wetness is expected to be the result of foam associate this level probe with the pump you assign to add antifoam see Section 11 1 for details about pump assignment When the foa...

Page 83: ...71 New Brunswick Scientific User s Guide 7 5 About Pump Calibration To assure the most accurate flow rate calibrate the pump each time you change tubing See Section 11 6 4 for details...

Page 84: ...to from the vessel and or cabinet at any time make sure the main water supply is closed CAUTION During sterilization the bearing housing cap must be installed on the fermentation vessel bearing housin...

Page 85: ...etrations that are immersed in the media 7 Remove the RTD from the thermowell 8 Disconnect all probes and sensors and remove their cables 9 If you are using pH and DO probes install each probe s short...

Page 86: ...the optional angled autoclave rack part number XMF 8624 M1227 9231 see Figure 31 below Secure it in place with the strap 2 If the vessel is not water jacketed skip to Step 3 If the vessel is water ja...

Page 87: ...ilable the autoclave should be on liquid cycle pressure release NOTE Filter manufacturers generally advise limiting filter sterilization to 30 minutes but the longer time required for slow exhaust is...

Page 88: ...Cooling Loop In and Cooling Loop Return between the cabinet and the vessel 4 Connect Exhaust In and Return between the cabinet and the exhaust condenser if present 5 Secure all connections 6 Connect...

Page 89: ...D Figure 33 Loading Pump Tubing 1 Open the PUMP cover to gain access to the interior of the pump 2 Select the desired tubing size see Table 6 in Section 11 4 for reference and cut a length sufficient...

Page 90: ...o compensate for any discrepancy you will need an accurate external pH meter 1 Following sterilization with the media at room temperature note the pH value on the BioFlo CelliGen 115 SUMMARY screen 2...

Page 91: ...nsult Table 6 the flow rate tubing size chart for guidance 9 4 1 Addition Tubing Size pH can be controlled by automatic additions of liquid acid and base Additions are triggered by the BioFlo CelliGen...

Page 92: ...as small as 0 2 mm sometimes eliminate overcontrol while supplying sufficient liquid during high demand culture phases NOTE Whatever the tubing ID the tubing wall thickness must be 1 16 inch 1 6 mm N...

Page 93: ...oxygen will not be controlled by means of a cascade Agitation controls dissolved oxygen through automatically controlled agitation speed When the actual DO2 value drops below the setpoint the system...

Page 94: ...ll has not reached the setpoint increasing the GasFlo entering through the sparger to as much as the high limit If the DO setpoint is still not achieved the cascade will begin to increase the O2 perce...

Page 95: ...se from 25 to 200 If there is still a need for more DO the GasFlo loop will kick in until the need is satisfied 2 Select the Cascade To loop or series of loops here Your selection will now have a dot...

Page 96: ...lk you through those operations There are three standard pumps on the front right of your control cabinet see Figure 32 repeated here for easy reference Figure 32 Standard Pump Array 11 1 Pump Assignm...

Page 97: ...ails on the choice of Level Wet and Level Dry see Section 11 6 1 11 2 Pump Setpoint To enter a setpoint for a pump 1 Open the PUMP screen Gauges for Pumps 1 3 are displayed in this screen see Figure 3...

Page 98: ...e providing more nuanced control of additions to maintain pH Contrary to the number shown in the Figure 36 Pump 1 Out Mult edit box we recommend that you begin by implementing a multiplier of 25 This...

Page 99: ...ed but its flow rate depends on the diameter of tubing you use Table 6 provides the pump flow rates according to various tubing diameters Table 6 Flow Rate per Tubing Size Tubing Wall Thickness 1 16 i...

Page 100: ...bration 11 5 Pump Period At the bottom of each pump gauge is the Period sec pane see Figure 38 Figure 38 Pump Period sec Use this edit box and its associated touchpad to enter a pump cycle time in sec...

Page 101: ...he vessel When the vessel is set up at the control cabinet raise the probe to the level at which you want addition to begin Never lower a probe after autoclaving 1 Open the PUMP screen 2 Select the fe...

Page 102: ...tact with the probe the pump is turned off when the liquid is in contact with the probe the pump is turned on 11 6 3 Level Control Off When OFF is selected from any level Foam HiFoam Lvl2 Wet Lvl 2 Dr...

Page 103: ...91 New Brunswick Scientific User s Guide NOTE To assure the most accurate flow rate calibrate the pump see Section 11 4 each time you change tubing...

Page 104: ...play first see Figure 39 If you find any other Setup screen in the display press the Controller Setup tab to open this screen Figure 39 Controller Setup Screen If you have more than one unit the dark...

Page 105: ...ol loops accordingly Although you can save each change one at a time in this screen by pressing it you can also wait until all changes have been selected If you leave this screen however and wish to s...

Page 106: ...manual gas mixing 1 rotameter with automatic gas mixing 1 TMFC with manual gas mixing or 1 TMFC with automatic gas mixing If your system is equipped with no TMFC or one TMFC the system will be preconf...

Page 107: ...2 and N2 3 as selected in Figure 41a or Air 1 O2 2 and CO2 4 The loops numbers 1 2 3 4 correspond to the gas connections on the cabinet see Figure 1g Figure 41a Air 1 Gauge Screen with 3 Gas This gas...

Page 108: ...onnections on the cabinet see Figure 1g Figure 41b Air 1 Gauge Screen with 4 Gas There is also a GasFlo loop when one TMFC is present settings in this loop s gauge screen turn the TMFC on and off and...

Page 109: ...mber and press the OK button 3 To change the month press the down arrow T and press the month you wish to select from its associated drop down menu 4 Press the Set button to save the new information Y...

Page 110: ...e system has the Base Power module the Main pH DO module and one TMFC This system is also set to NBS Modbus communication mode see the SCADA pane in Figure 44 and has the Unit ID number of 2 This is t...

Page 111: ...e will appear at the top of the screen 4 Each unit needs a unique ID number in the SCADA pane assign the correct Communication Mode and Unit ID number then press the Set button Figure 45a Adding New H...

Page 112: ...powered on 2 Press the SETUP button to open the SETUP screen then press the Hardware Setup tab see Figure 44 3 Press the Scan Hardware button and wait until all items are listed in the Unit panes on...

Page 113: ...mp 9 Enter the pump setpoint and press the ON button 10 Remove the water saline solution from the vessel 11 Add medium to the vessel 12 Ensure that all appropriate sensors and feed harvest tubes inclu...

Page 114: ...be connected for a minimum of six hours to be properly polarized before it can be correctly calibrated 11 Calibrate the DO probe see Section 7 3 12 Set pH and DO to the desired setpoints 13 Set the pH...

Page 115: ...t against the gasket 2 Close the valve on the sampler tube if it is open 3 Squeeze the bulb and holding it compressed tighten the sample bottle against the gasket 4 Open the valve and gradually let go...

Page 116: ...example approximately 2 3 hours Eventually however you must expect your batch to decline 13 6 4 Decline Phase This final phase is marked by a slow dying off which is of course inevitable 13 7 Batch O...

Page 117: ...arvested The fermentation vessel contains at all times the optimum amount of media with an established thriving culture Figure 47 Continuous Operation 13 10 Anaerobic and Microaerophilic Culture When...

Page 118: ...as mixture is dependent on the particular organism that you are culturing 13 11 Harvesting Procedure When the vessel is set up on the control cabinet adjust the level probe s tip to the level at which...

Page 119: ...07 New Brunswick Scientific User s Guide 6 Remove drain and clean the vessel as outlined in Section 15 See also Section 21 7 5 for shutdown and cleaning tips NOTE Never wash the filters or get them we...

Page 120: ...exhaust filter s is are not wet or clogged Also loosen the inoculation diaphragm cap for additional venting After autoclaving tighten the inoculation cap When the vessel is installed on the control ca...

Page 121: ...em 1 Attach a Y fitting to the top of the condenser with a piece of tubing Be sure to secure the tubing with a tie at each end 2 Attach an exhaust filter to each branch of the Y This allows you the fl...

Page 122: ...e several times with tap water 4 Repeat rinsing with distilled water and let it dry 15 1 1 List of Wetted Parts For further reference in your choice of cleaning detergents Table 7 provides a list of w...

Page 123: ...t with the electrode tip immersed in a solution of 3 molar KCl or a buffer solution between pH 4 00 and pH 7 00 CAUTION Never let a pH probe rest on its tip Never leave a pH probe in DI water 16 2 DO...

Page 124: ...and bolts to make sure they are properly tightened Tighten any loose item s 2 Check that all controls and connectors are free of dust 3 Check that all O rings in the headplate and impellers are intac...

Page 125: ...s representative Heaters Heater Blankets 1 3L 3 0L M1369 3107 Water Jacket Heaters 7 5L 14 0L M1369 3108 1 3L M1369 8021 3 0L M1369 8022 7 5 M1369 8020 Heat Blankets 14 0L M1369 8023 Motors Direct Dri...

Page 126: ...easily and quickly yourself If the problem is not listed below or if the suggested solutions do not work please call your NBS representative to request a service technician Other than the solutions pr...

Page 127: ...ecure connection Replace probe cable or DO probe pH probe readings are erratic Recalibrate the probe carefully following instructions in this manual Check for a secure connection Gel filled probe may...

Page 128: ...e Clamping Ring 28 10 Water Jacket Guard Installation top view 29 11 pH Probe with Port Adapter exploded view 34 12 dO2 Probe with Port Adapter exploded view 36 13a Exhaust Condenser 1 3L 3 0L 7 5L ve...

Page 129: ...t 86 37 Calibrating the Pump Flow Rate 88 38 Pump Period sec 88 39 Controller Setup Screen 92 40 Changing Operating Control Mode 93 40a Operating Control Mode Changed 93 40b Changing Vessel Size 94 41...

Page 130: ...actuators that maintain the various parameters at their setpoints Using temperature as an example the controller compares the output of a temperature sensor to the user entered temperature setpoint a...

Page 131: ...n include proportional P integral I and derivative D terms The P I and D constants are three numbers that determine the relative sizes of the proportional integral and derivative terms respectively To...

Page 132: ...ms this task A number of textbooks1 that explain the theory and describe the process could be useful for the mathematically inclined novice The Ziegler Nichols method described in the footnoted refere...

Page 133: ...normalizing constant for the loop The controller reevaluates SN every second I is divided by 60 so any value entered by the user should be in reciprocal minutes The normalizing constant k can be set t...

Page 134: ...C Agitation 1000 RPM Aeration 1 VVM 20 1 1 OTR Calculations OTR can be estimated by titrating a fixed amount of sodium sulfite Na2SO3 with air CU 2 2SO3 02 2SO4 The Procedure Calibrate the DO electro...

Page 135: ...115 has a 3 24 inch 0 015 diameter If three impellers are to be used 3 06 diameter impellers are required This size impeller is normally used in a 7 5L BioFlo CelliGen 115 vessel These impellers shou...

Page 136: ...low rate of one vessel volume per minute VVM may produce as much as 40 reduction in the horsepower used It is required that some air gas flow be utilized when running at speeds above 750 RPM The relat...

Page 137: ...CelliGen 115 the information is generally applicable for any fermentation 21 1 Media Formulation Question What kind of media should be used and does it differ from media used in shake flasks Answer T...

Page 138: ...ml L When the foam probe is used the pumping of antifoam is controlled by the unit The pump should be set to add the minimum amount of antifoaming agent required to prevent foaming in your particular...

Page 139: ...ert the needle though a septum port so that the drip point is away from stainless steel components and fairly close to the liquid level You may also use a more diluted solution of the acid or base How...

Page 140: ...position If the glass wool is going to be replaced for the run then remove it and the rubber sampler bulb prior to rinsing The protective bearing housing cap must also be in place It will be necessar...

Page 141: ...wish to use the newer polymer clamps during the running of your fermentation then place them onto the tubing but leave them open Use easily removable metal clamps to actually close the line during au...

Page 142: ...in fact the caps should always be on except when the probe is being hooked up to the unit NEVER autoclave a pH probe or a DO probe without its protective cap Check the DO probe to be sure the require...

Page 143: ...t the water lines connecting the outgoing return lines before the incoming delivery lines and ensuring that the delivery and return lines are not inverted Insert the temperature probe into the thermow...

Page 144: ...down Additional media components that are not autoclaved can be added once the vessel has cooled sufficiently The protocol for this is the same as for inoculation as described below Inoculation can be...

Page 145: ...in such a way as to protect the sensitive tip Removing the pH probe is usually not so difficult inserting it because the shaft is wet and should be relatively easy to remove The danger of probe break...

Page 146: ...rt beveled tips of the interior portion of the base type addition ports will often require special attention All tubes and shafts must be cleaned Note that there may be some residual base or acid left...

Page 147: ...l Products Corporation catalog number 6732 Alternatively if you are using the vessel for consecutive runs with the same media rinsing it with warm tap water and with DI water may suffice Note that if...

Page 148: ...Guide 2 22 2 A AP PP PE EN ND DI IX X D D C CO OR RR RO OS SI IO ON N R RE ES SI IS ST TA AN NC CE E Websites such as www outokumpu com provide up to date information about the 316 type stainless ste...

Page 149: ...ood Resilience Rebound Good Abrasion Resistance Good Tear Resistance Fair Solvent resistance Poor Oil resistance Poor Low Temperature Usage 20 to 60 F 29 to 51 C High Temperature Usage to 350 F 177 C...

Page 150: ...nd 8 45 46 103 BioFlo 115 Options Setting the 100 Bottom Clamping Ring Installing the 28 C Cabinet Cleaning of 110 Calibration of Touchscreen 50 97 Calibration Screen 58 Cascade Creating a 82 Cascade...

Page 151: ...Fermentation Run Phases of 103 Preparing for 101 Fermentation Techniques 125 Fermentor Information Sheet vii Filling the Water Jacket 29 Foam Control 7 101 Foam Exhaust Tube Installing the 33 Foam Le...

Page 152: ...t Multiplier 86 P P I Gains 66 Parts Lists 113 pH Control of 6 pH Probe 6 Calibration of 62 Inspection of 62 Installation of 34 64 Maintenance of 65 Storage of 65 PID Explanation of Constants 119 Expl...

Page 153: ...ntrol 6 RTD 6 Setpoint 6 Temperature Probe Installation of 80 Thermowell Installation of 32 Touchscreen Calibrating the 50 97 Troubleshooting 114 Tubing Recommendations 126 Tubing Size 126 U Unused Po...

Page 154: ...142 BioFlo CelliGen 115 y M1369 0050 User s Guide...

Reviews:

No comments

Brands by name

Popular brands

Load more brands