103
New Brunswick Scientific
User’s Guide
If you prefer to inoculate via an addition port, be sure to flame the connectors and use
an inoculum flask as your “addition vessel” (
see Figure 34 for reference
).
13.4 Start
BioCommand (if present)
1.
Start the
BioCommand
supervisory software on your computer, reset the EFT
(Elapsed Fermentation Time) to zero, make appropriate program selections to
begin logging data.
2.
Make sure all gas pressures are 10 PSI and the water pressure is 10 PSI.
3.
If your BioFlo/CelliGen 115 has rotameter air flow control, adjust the airflow to
the desired rate. Check to see that flow is stable and that all gases are properly
connected.
13.5 Sampling
Procedure
Referring to Figure 14a or 14b, whichever represents your sampling system:
1.
Check to be sure that the sample bottle is slightly loose, not tight against the
gasket.
2.
Close the valve on the sampler tube, if it is open.
3.
Squeeze the bulb and, holding it compressed, tighten the sample bottle against the
gasket.
4.
Open the valve and gradually let go of the rubber bulb to obtain the desired sample
volume.
5.
When you have obtained the desire volume, close the valve.
6.
Unscrew the sample bottle from the sampler. Take the cap from a new bottle, and
place it on the sample-filled bottle.
7.
Install the new bottle in the sampler and make sure that the sample bottle is firmly
sealed against the sampler gasket.
Always use aseptic techniques.
8.
Repeat the above steps until you have the desired number of samples.
13.6 Fermentation
Phases
In a typical fermentation run, you can expect to see four characteristic phases: (1) the
Lag phase, (2) the Exponential Growth phase, (3) the Steady State phase, and (4) the
Decline phase.
13.6.1 Lag
Phase
This initial phase is aptly named because it is the slow beginning of your
fermentation run, while the microbes become accustomed to their medium.
