108
BioFlo/CelliGen 115
y
M1369-0050
User’s Guide
1
1
4
4
E
E
S
S
S
S
E
E
N
N
T
T
I
I
A
A
L
L
O
O
P
P
E
E
R
R
A
A
T
T
I
I
N
N
G
G
T
T
I
I
P
P
S
S
14.1
Precautions for Glass Vessel Assembly
There are certain precautions you should take to avoid cracking or breaking the glass
vessel during assembly and autoclaving:
•
Glass can crack or break during assembly if the clamping screws are
overtightened. As a precaution, tighten the screws only finger tight prior to
autoclaving. You should be able to insert a business card between the glass and
the metal.
•
If the vessel is not sufficiently vented during autoclaving, it can crack or break. As
a precaution, make certain that the exhaust filter(s) is (are) not wet or clogged.
Also loosen the inoculation diaphragm cap for additional venting.
•
After autoclaving, tighten the inoculation cap. When the vessel is installed on the
control cabinet and air is freely flowing through it, you may retighten all nuts and
screws, again taking care not to overtighten.
#
NOTE:
To maintain the best possible seal, O-rings should be replaced every six
months or more frequently if needed.
14.2
Exhaust Condenser & Exhaust Filters
The inner assembly of the exhaust condenser can be removed for cleaning:
1.
Pass warm water and detergent through the top of the condenser, but not through
the quick-connects. Do this twice.
2.
Run clear water through once.
3.
Blow out with air.
4.
Autoclave.
Clean the exhaust condenser after each run. This is most critical when operating as a
chemostat for protracted fermentation times.
