2
BioFlo/CelliGen 115
y
M1369-0050
User’s Guide
1.2 CAUTIONS
CAUTION
Page
This equipment must be operated as described in this manual.
If
operational guidelines are not followed, equipment damage and personal injury
can occur. …
Do not use this equipment in a hazardous atmosphere or
with hazardous materials for which the equipment was not designed.
iv
Before making electrical connections,
verify that the supply voltage matches
the voltage and the power requirements marked on the electrical specification
plate …and the control schematics supplied with the unit.
12, 15
Make sure all utility connections have been securely made before
connecting to WATER-IN and before turning on the main water supply.
15
Before connecting or disconnecting the water hoses to/from the vessel
and/or cabinet at any time, make sure the main water supply is closed.
16, 72,
102
To protect the integrity of your glass vessel and to avoid damage,
familiarize yourself with these cautions
…
18
…do not fill the water jacket or operate the vessel until you have cut the
cable ties and released the stir bar.
26
Finger tighten only any adapter that has a white Teflon ferrule
(tapered,
cone-shaped insert under the Teflon washer). The ferrule can deform under too
much pressure.
31
Make sure that the thermowell does not touch the cooling coil.
32
Do not install the pH port adaptor in the headplate before inserting the
probe
...
34, 64
Do not install the dO2 port adaptor in the headplate before inserting the
probe
…
35, 67
To avoid vessel stress cracks,
especially during autoclaving, make vessel
clamping screws finger tight.
43
Before turning on the main power switch
, make sure that: (1) the input water
hose is connected, the drain line is connected and the water supply is turned on;
(2) the vessel is in place and the quick-connect water lines are connected to the
vessel’s heat exchanger; (3) the power cord is properly connected to the control
cabinet and plugged into a suitable power outlet.
45
Be sure to wear protective gloves
when installing a glass electrode.
63
We recommend that you avoid the use of hydrochloric acid (HCl)
with the BioFlo/CelliGen 115 for pH control or any other purpose, because HCl
corrodes stainless steel. Over time, it will severely damage the headplate, a
costly component to replace, and other stainless steel components.
Phosphoric and sulfuric (10% maximum concentration) acids are acceptable and
are commonly used for pH control.
65
Never let a pH probe rest on its tip,
and never leave a pH probe in DI water.
65, 111
During sterilization: the bearing housing cap must be installed on the
fermentor bearing housing
, to keep steam from damaging the internal
bearings.
72
Never autoclave PVC tubing
(clear with white braiding).
72
...continued...
