65
Leica ST5020
Setting the dips
• Press
DIP
to switch from
yes
(= Dips activated) to
no
and vice versa.
• Duration and speed of one complete up and down movement (dip) are
selected in the
MOVEMENT
menu. The parameters selected there will
be applied to all programs. Increasing numbers of dips reduce instru-
ment throughput.
For exact steps the following applies:
If the immersion time of an exact step is shorter than the time to do
the set number of dips, only the number of dips that fit into the im-
mersion time will be done.
Defining exact steps
As there is potential for timing clashes to occur when multiple
racks are present in the instrument, steps which require precise
timing are designated as 'exact' in the program.
• Press
EXACT
to alter between
yes
(=exact) and
no
(not exact).
• Immersion times at exact steps are achieved to within ± 1 second.
Immersion time at non-exact steps is at least as long as defined, most
of the time however, non-exact steps take longer than defined, as
racks due for non-exact steps receive attention only as the transfer
arm becomes available after an exact step.
• Define 'exact' steps only when really needed. The more exact steps,
the slower and less flexible the instrument (instrument throughput de-
creases).
If you interrupt the staining with PAUSE, take care not to do so
while an exact step is in progress. Otherwise, the program will be
stopped and the duration of the step will be extended by the dura-
tion of the pause. This can the cause errors during staining.
If PAUSE buttun has been pressd druring an exact step is in pro-
cess, a warnig message will be appear in the dialog box
5. Operation
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