Biotek MicroFlo select, Operator'S Manual

Page: 94 / 130

76 | Instrument Qualification
BioTek Instruments, Inc.

The final absorbance for all dye solution concentrations should be in
a range between 0.700 and 1.300 OD.

Using pure DI water in place of these solutions is not recommended
and will likely result in the failure of the unit to meet specifications.

Liquid Test Worksheet templates can be found at the end of this
chapter for recording data reduction results. If your tests are failing,
this information will be useful for BioTek TAC to help diagnose any
problems.
Test Solutions
Unique concentrations of the test fluid are described here, each one corresponds to
a specific dispense volume, except the 1536 Solution for 1536-well testing. Prepare
the solutions you will need to validate the cassette types and dispense volumes
used most commonly in your applications.

The 5 µL Solution is used to make the higher volume test solutions.
1 µL Solution
Using BioTek’s 10X concentrated blue dye solution (PN 7773001), mix 5 mL
of DI H2O with 8 mL of the blue dye solution.
5 µL Solution
Using BioTek’s 10X concentrated blue dye solution (PN 7773001), mix 100
mL of DI H2O with 10 mL of the blue dye solution.
10 µL Solution
Mix 25 mL of DI H2O with 20 mL of the 5 µL Solution (described above).
50 µL Solution
Mix 45 mL of DI H2O with 5 mL of the 5 µL Solution (described above).
100 µL Solution
Mix 40 mL of DI H2O with 2 mL of the 5 µL Solution (described above).
1536 Solution
Mix 5 mL of 70% Isopropyl Alcohol with 3 mL of the
5 µL Solution
(described above) and 35 mL of DI H2O.