Biotek MicroFlo select, Operator'S Manual

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Dispense Precision and Accuracy Tests
|
75
MicroFlo Select Microplate Dispenser
well of the plate. It dispenses 10

L into each well using the 10

L Solution ,
and requires an additional 150 µL of DI water to raise the fluid level for optimal
reading.

50 µL Test : The 50

L test is used to confirm the performance of the 5

L
cassettes when dispensing 10 aliquots (2 1/2 turns of pump) into each well of
the plate. Dispense 50

L into each well using the 50

L solution , and requires
an additional 100 µL of DI water to raise the fluid level for optimal reading.

100 µL Test : The 100

L test is used to confirm the performance of the 10

L
cassettes when dispensing 10 aliquots (2 1/2 turns of pump) into each well of
the plate. Dispense 100

L into each well using the solution called 100

L
solution , , and requires an additional 50 µL of DI water to raise the fluid level
for optimal reading.

1536 Test : This test is intended to check the alignment of the fluid jets
produced by the 1536 well cassettes. Given the small diameter wells of the 1536
well plates, it is important to confirm that the tips in the cassette are firing
straight into the wells. Dispense 6

L into every other column of a 1536 well
plate using the solution called 1536 solution . Then, perform the 1 µL Test
described above.
Materials

Corning Costar #3590 96-well plates, or equivalent

1536-well plates (when testing 1536 models), Nunc #264710

Precision balance with readability of 0.0001 g resolution is preferable, 0.001 g
resolution is acceptable, and capacity of 100 g minimum

Pipettes and graduated beakers

Microplate absorbance reader capable of dual wavelength reading at 630 and
405 (or 450) nm

If you are using one of BioTek’s keypad-based absorbance readers,
such as the ELx800™ or ELx808™, ensure that the reader is not
running in Rapid mode. To check this, select UTIL > READ and cycle
through the prompts until you see READ IN RAPID MODE?”

BioTek blue dye solution, PN 7773001, or equivalent to create the test solutions
described on the following page. See also the important notes below:

BioTek determined the pass/fail specifications for the following tests
using the test solutions described. You may choose to use your own
solutions. If any tests fail using your own solutions, however, retry the
tests using the suggested solutions.

The absorbance of blue dye solutions should be measured at 630/450
(or 405) nm. The BioTek blue dye solution part number is 7773001.