78 | Instrument Qualification
BioTek Instruments, Inc.
7.
Put the Tube Organizer into the test fluid vessel and
Prime
the tubing until
any large air bubbles are removed.
8.
Run
the dispense protocol.
9.
Place the plate on the balance and record the
Total Dispense Weight
in the
worksheet.
10.
Using a calibrated hand pipette, add the specified amount of de-ionized water
to each well to raise the fluid level to approximately 150
L, except for the 1536
Test:
0.
11.
Read the plate in an absorbance reader using the dual-wavelength method:
read the plate at 630 nm and 450 nm.
If you are using one of BioTek’s keypad-based absorbance readers,
such as the ELx800™ or ELx808™, ensure that the reader is not
running in Rapid mode. To check this, select UTIL > READ and cycle
through the prompts.
12.
Calculate the
Delta OD
: (630nm – 450nm),
Mean Absorbance
,
Standard
Deviation
, and the
%CV
for the wells under test.
%CV
= (Standard Deviation
Mean) * 100.
13.
Print the report, obtain required signatures, and store it according to regulatory
guidelines.
0.
If one or more of your tests are failing, make sure the dispense tubes are not clogged,
(follow the instructions for Unclogging the Tips in the
Maintenance
chapter). If that
doesn’t work, recalibrate the cassette and repeat the test(s). If your tests continue to
fail, contact BioTek’s Technical Assistance Center (TAC).
Test
Volume
1 µL
150 µL
5 µL
150 µL
10 µL
100 µL
50 µL
100 µL
100 µL
50 µL
1536
0 µL
Summary of Contents for MicroFlo select
Page 1: ...Operator s Manual Dispenser MicroFlo Select ...
Page 2: ......
Page 44: ...26 Installation BioTek Instruments Inc ...
Page 74: ...56 Operation BioTek Instruments Inc ...
Page 116: ...98 Dispensing to Test Tubes BioTek Instruments Inc ...
Page 126: ...108 Gravimetric Calibration Instructions MicroFlo Select Microplate Dispenser ...