About Dispense Protocols
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MicroFlo Select Microplate Dispenser
Dispense Parameters
height by entering the number of steps or millimeters.
Horizontal
Position
From a central axis of 0, you can adjust the plate position to
dispense to one side of the well. This may be useful for some
cell-based assays. Some trial and error is most likely needed
to find the optimal position.
Increase the number of steps to move the plate to the left,
so the dispense tips address the right side of the wells.
Decrease the number of steps, using a negative number, to
move the plate carrier to the right, so the dispense tips
address the left side of the wells.
For all plate types, the default value is 0 (zero) for the
horizontal offset.
Using the keypad: With the number of steps set to 0, hold
the Shift key and press Options to enter a negative
number.
Using the LHC: Click
Show advanced
options and use the
text field to enter the number of steps, or use the scroll
keys. The corresponding distance in millimeters is displayed.
Prime Before
Start?
Select Yes to prime the tubing before dispensing to the
plate, then define the prime values. Otherwise, select No to
not perform a prime before dispensing.
Prime Volume
Enter the amount of fluid in microliters (µL) to pump through
each tube into the priming trough per prime cycle. The
recommended prime volume is 10 times the cassette size:
10 µ
L
for 1 µ
L
cassette; 50 µ
L
for 5 µ
L
; 100 µ
L
for 10 µ
L
.
Number Pre-
Dispenses
Enter the number of prime cycles to perform before
beginning the dispense.
Important: Priming the dispenser after it has been idle for 20 or more
minutes, especially when using the 1 µL cassette, is essential for
achieving accurate dispense volumes. Press the Prime button on the
dispenser or add a Prime to an LHC protocol.
Protocol Names
Onboard the MicroFlo, protocols are tracked by a number because the keypad does not
offer a way to easily search for a protocol by its name. (The opposite is true when using
the LHC computer control.) Up to 99 Protocols can be saved onboard the dispenser.
(And, unlimited number can be saved on your PC.)
The MicroFlo assigns the first available number to a newly created protocol. The
dispenser ships without any pre-defined protocols, so the first one you create will be
numbered 01. The second will be numbered 02, and so on.
Summary of Contents for MicroFlo select
Page 1: ...Operator s Manual Dispenser MicroFlo Select ...
Page 2: ......
Page 44: ...26 Installation BioTek Instruments Inc ...
Page 74: ...56 Operation BioTek Instruments Inc ...
Page 116: ...98 Dispensing to Test Tubes BioTek Instruments Inc ...
Page 126: ...108 Gravimetric Calibration Instructions MicroFlo Select Microplate Dispenser ...