Biotek MicroFlo select, Operator'S Manual

Page: 95 / 130

Performing the Precision and Accuracy Tests
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77
MicroFlo Select Microplate Dispenser
Performing the Precision and Accuracy Tests
Prerequisite:

Prepare the test solutions as described on page 76.

Make a copy of the applicable worksheet beginning on page 80.
Procedure:
1. Install the cassette to be tested.
2. Turn on the MicroFlo Select and make sure the cassette type setting is correct.
3. Turn on the balance.
4.
Fill a beaker or other vessel with the test solution.
5. Define a Protocol and save it for reuse. Set the parameters based on the desired
test volume:

Set the Dispense Volume to match the Test:

Set the Flow Rate to:

1 µL cassette = Medium

5 µL cassette = High

10 µL cassette = High

Retain the default settings for Dispense Height, Plate Clear Height and
Horizontal Position.

Define a Prime before start : set the volume to 10 µL.

Set the Number of Pre-dispenses to 2.

1536 Test only: : Change the plate map to dispense to every other column.
It will be easier to observe the dispense results than dispensing to the whole
plate.
6. Place a clean/new microplate on the balance and tare the balance.
Test Volume
1 µL 1 µL
5 µL 5 µL
10 µL 10 µL
50 µL 50 µL
100 µL 100 µL
1536 6 µL