Biotek MicroFlo select, Operator'S Manual

Page: 92 / 130

74 | Instrument Qualification
BioTek Instruments, Inc.
Liquid Testing Methodology
The tests are fundamentally the same for all cassettes except the 1536: Tare an empty plate
on a balance. Use the MicroFlo Select to dispense a quantity of fluid with a known dye
concentration to the wells. Weigh the plate to obtain the weight of the fluid dispensed.
Pipette deionized water on top of the dye to bring the wells up to a more optically
measurable volume. Read the wells in a microplate reader and determine the percentage
Coefficient of Variance (%CV) among all wells, and the accuracy of the volume dispensed
in each well (% Accuracy Error).
1 µL 1536 Cassettes : To test the 1536 cassette type, dispense the 1536 Test Solution to
every other column of a 1536-well plate. Examine the wells to determine if the cassette is
dispensing precisely to the selected columns with no splatter into non-selected columns or
onto the plate surface. Then, perform the tests defined for the
1 µL cassette type using a 96-
well plate.
BioTek recommends performing two tests, one at the volume that matches the cassette
type and another that best represents the cassette type and dispense volume most common
to your applications:
Cassette Types
Tests –
Solutions
1 µL 1 µL 1536 5 µL 10 µL
1 µL
√ √
5 µL
√
10 µL
√ √ √
50 µL
√
100 µL
√
1536 µL
√

1 µL Test : The 1

L test is used to confirm the performance of the 1

L
cassettes when dispensing a single aliquot (1/4 turn of pump) into each well of
the plate. It dispenses 1

L into each well using the 1

L Solution , and requires
an additional 150 µL of DI water to raise the fluid level for optimal reading.

A single aliquot for a cassette type is the smallest volume unit
recommended for it. 1µL for the 1µL cassette, 5µL for the 5µL
cassette, and 10µL for the 10µL cassette.

5 µL Test : The 5

L test is used to confirm the performance of the 5

L
cassettes when dispensing a single aliquot (1/4 turn of pump) into each well of
the plate. It dispenses 5

L into each well using the 5

L Solution , and requires
an additional 150 µL of DI water to raise the fluid level for optimal reading.

10 µL Test : The 10

L test is used to confirm the performance of the 1

L
cassettes when dispensing 10 aliquots (2 1/2 turns of pump) and the 10

L
cassettes when dispensing a single aliquot (1/4 turn of the pump) into each