Biotek MicroFlo select, Operator'S Manual

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Calibrating the Tubing Cassette Gravimetrically
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101
BioTek Instruments, Inc.
Figure 1: A MicroFlo Select in preparation for the gravimetric cassette calibration, and a digital
precision balance with 0.0001 g resolution.
Procedure:
1 Install the cassette to be calibrated.
2 Turn on the MicroFlo Select and make sure the cassette type setting is correct.
3 Turn on the balance.
4 Define a Protocol with the following parameters:

Set the Dispense Volume to match the cassette:

Set the Flow Rate to:

1 µL cassette = Medium

5 µL cassette = High

10 µL cassette = High

Retain the default settings for Dispense Height and Horizontal Position

Define a Prime before start : set volume to 10 µL.

Set the Number of Pre-dispenses to 2.
Cassette Volume
1 µL 10 µL
5 µL 50 µL
10 µL 100 µL