BIO RAD 145-1001, Instruction Manual

Page: 100 / 120

S3 Cell Sorter Instruction Manual 90 |
If sonication of the tip is not performed:
10. Using a 1 ml syringe filled with ethanol, press against the top and eject ethanol through the
nozzle tip, rotating between the 100 µm tip and the larger opposite end to free clog.
11. Watch the resulting stream. The fluidic stream should be coming out straight from the tip.
12. Repeat if necessary until the stream is coming out straight.
13. Proceed to step 14.
14. Place the O-ring at the point on the nozzle where the bottom of the black collar will be.
15. Place the nozzle tip back onto the nozzle assembly by pushing it up using the sides of the
tip. Avoid touching the end.
16. Secure the black retaining collar until finger tight.
17. Remove the lint-free cloth from nozzle chamber.
18. Press the stage back down and tighten the thumb screw.
19. Click the blue forward arrow.
Fig. 101. Nozzle swap wizard window.
Fig. 100. Nozzle swap wizard window.