BIO RAD 145-1001, Instruction Manual

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Table 20. Instrument controls and their functions.
Button Name Function
Start Sample Starts sample acquisition. If a data file is loaded and it has been saved,
Acquisition the data will clear and acquisition will start
Stop Sample Stops acquisition and sample flow. Regions, gates, and annotations may
Acquisition be modified before saving the FCS file
Start Sorting Starts sorting. If a data file is loaded and it has been saved, the data will
clear and sorting will start
Stop Sorting Stops sorting and sample flow. Regions, gates, and annotations may be
modified before saving the FCS file
Clear Acquired Data Clears acquired data. All plots and statistics will be refreshed.
Pause Sample Pauses sample acquisition and sample flow. The data at this point may be
appended (by the Resume function) or saved as is. Saving after pausing will
disable the Resume function. To remove sample tube, unlock the touchscreen
locking system located above the loading stage after pausing acquisition
Resume Sample Resumes acquisition when a sample has been paused. Resuming will
append the paused FCS file and the time paused can be seen on a time
histogram. If you have removed the sample tube it must be returned and
relocked before acquisition can be resumed
Eject Sample Tube Depressurizes and unlocks the sample. The loading stage can be moved
into wash position to access sample. The touchscreen locking system can
also be used to unlock the sample
Adjust Sample Starts or stops agitation of the sample. There are three levels of agitation:
Agitation low, high, and off. Click this button once to set to low, twice to set to high,
and three times to turn off agitation
Auto Save When checked, automatically prompted to save a data file after
acquisition stops.
Target Event Rate Allows selection of target event rate for sample acquisition or use of preset
sample rates low/med/high based on sample concentration
Limit When checked, stops data acquisition after the number of events entered
has been reached
Gate Limit When checked, data acquisition stops after the number of events entered
has been reached within the selected gate
Cycle Mode When checked, automatically refreshes acquired data at the entered time
Sample Chamber Turns the sample chamber light on and off
Light
Enable/Disable Sort Illuminates entire sort collection chamber for easy viewing of deflection
Chamber Light plates, sort stream, and collection vessels
Enable/Disable Enables or disables temperature control of the sample station and sort
Temperature Control collection vessel
Biosafety Indicates the status of the biosafety system and gives information on filter life.
System Status If the biosafety system loses connection, cannot connect, or if the filter life is
at 0% an error will occur and not allow the user to sort or acquire.
continues