BIO RAD 145-1001, Instruction Manual

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| 105 S3 Cell Sorter Instruction Manual | 105
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S3 Cell Sorter Specifications
The specifications for the S3 system are shown in Table 30.
Table 30. S3 cell sorter specifications.
Performance Acquisition rate 100,000 events per sec
Sorting rate 30,000 events per sec
Sorting purity >99% pure
Sensitivity <125 MESF for FITC and PE
Temperature control Sample and collection temperature control from 4–37°C
Optics Lasers Primary: 488 nm 100 mW
Optional: 561 nm 100 mW and 640 nm 100 mW
Detection Forward scatter (FSC) with PMT
Side scatter (SSC) with PMT
4 fluorescent detectors with PMT
Fluidics Nozzle 100 µm fixed
Pressure 30 psi fixed
Sheath Onboard dilution of 8x sheath or direct use of 1x sheath
Automation Auto alignment
Auto drop delay calibration
Auto droplet monitoring
Auto stream to laser alignment
Sorting Directions Two way
Modes Single, purity, enrich
Collection Up to 5 x 5 ml or 1.5 ml sample tube each direction
Microscope slides
2 x 8 micro well strip each direction
Software
Program ProSort
™
Workstation Computer requirements 5 Quad Core; 8 GB RAM; 250 GB HDD, Windows 7 Pro x64
monitor Recommended 24” LCD; 1920 x 1080 resolution
Physical requirements Dimensions (W x D x H) 70 x 65 x 65 cm deep (instrument only)
Weight 90 kg (instrument only)
Electrical 120–230 VAC; 50–60 Hz
Temperature Ambient temperature range of 18–25°C
Noise <60 dBA while in operation
Options Biosafety cabinet S3 biosafety system Class I
Regulatory status Electrical Electrical Safety for Europe
IEC 61010-1 CB Scheme
UL/CSA Standard for Safety Electrical Equipment for
Electrical Safety (USA, Canada, NRTL)
IEC61010-2-081, EN 61010-2-081
EN/IEC 60825-1, EMC EN 61326-1
CE Declaration of Certification
Laser Class I (1) laser product per CDRH regulations and
EN/IEC 60825-1