BIO RAD 145-1001, Instruction Manual

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36 | S3 Cell Sorter Instruction Manual 36 |
During the startup process, the fundamental alignment window will also open to initiate
cameras, maintain droplets, and align streams to the lasers. The startup process also includes
a warm up period (Figure 34).
After startup completion, both windows will close automatically and buttons in the toolbars will
become available to use.
Note: Automated startup is an option for this instrument. At shutdown, a startup time can be
designated. If this option is selected, be sure to empty the waste container and fill the sheath
fluid and DI containers at the end of the day.
Note: To change the fluidic containers after the software has been initiated or during a run, refer
to Section 5.3, Checking or Swapping Fluidics, for instructions on performing a hot swap.
4.4 Quality Control
The quality control (QC) procedure must be run every day to ensure optimal system
performance. This procedure requires the ProLine
™ calibration beads to adjust alignment, verify
system against the QC criteria, set up droplet creation, adjust side streams, calculate drop
delay, and maintain the droplet break-off.
Note: ProLine Universal calibration beads must be used on a system with a 488/640 nm lasers.
These beads can also be used on the 488 nm or 488/561 nm systems.
To perform the quality control procedure:
1. Vortex a bottle of ProLine calibration beads.
2. Place at least 10 drops (approximately 0.5 ml) into
a 5 ml tube. Do not dilute these beads.
3. Place the tube with beads into the sample input
station.
4. Move the loading stage into the run position.
5. Click
Run QC on the Setup and Maintenance tab.
6. Choose the correct bead lot from the dropdown
menu in the Event Based Alignment Settings
window (Figure 35).
Fig. 34. Startup Process window.
Fig. 35. Event Based Alignment
Settings window.