BIO RAD 145-1001, Instruction Manual

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S3 Cell Sorter Instruction Manual 94 |
10.5 Optical Filter Cleaning
The optical filters in the S3 system will lose performance when dirt, dust, or fingerprints are
present on the glass surface. Inspection of these optical components and regular cleaning will
help maintain the high performance of the system. Always remember that these coated pieces
of glass are delicate and should be handled with care. Any scrape or scratch on the surface
could significantly affect the light passing though.
To clean a filter, first put on gloves to avoid depositing oils and particulates on the surface.
Using compressed air, gently blow off the surfaces of the filter to remove any large debris
particles. Then, using a lint-free wipe (lens paper) or swab moistened with isopropyl alcohol,
gently wipe the surfaces of the filter. Inspect filter by holding up to a light to ensure all debris
particles have been removed. Replace filter into the S3 system.
10.6 Disinfectants
Always follow the PPE guidelines relevant to your laboratory’s safety procedures for dealing
with the disinfectants recommended below.
Disinfectants for Use in Sample Line
n
70% ethanol in DI water
n
Bleach solution with a maximum concentration of 5,800 ppm active chlorine, a 1:10 dilution,
which is roughly equivalent to 10% active chlorine. The bleach solution may be diluted further,
depending on the pathogenicity of the sample
Disinfectants for Use in Sheath Line
n
Bleach solution containing 580 ppm active chlorine, a 1:100 dilution, which is roughly
equivalent to 1% active chlorine
Disinfectants for Use in the Waste Container
The following disinfectant types may be used in the waste container. It is imperative that
the appropriate type and quantity of disinfectant is placed in the waste container to ensure
effective inactivation of the biologics in the tank. Please check compatibility of combined
products before use.
n
10% sodium hypochlorite or bleach
Fig. 109. Calibrating Droplets and preparing from drop
delay determination.