BIO RAD 145-1001, Instruction Manual

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4.5.8 Histograms and Density Plots
Histograms and density plots can be created from the Home tab. Clicking on each button will
create the plot and add it to the protocol workspace. Each plot will be added to the right of
the prior plot and will wrap to the next row as needed. Plots will be created with the default
parameters of FSC for histograms, and FSC and SSC for density plots. Refer to Section 3.3.8,
Global Preferences, for information on how to change plot defaults for axis, log vs. linear, plot
size, and plot display options.
4.5.9 Resizing, Moving, and Deleting Plots
When plots are added to the workspace they are tiled automatically based on the size of the screen.
To resize histograms or density plots:
1. Hover over the desired plot.
2. Hold down the handle in the lower right corner and drag the plot to the desired size.
4.5.6 Saving FCS Files
When the AutoSave box is not checked, the FCS file can be saved after acquisition or sort. The
protocol used during the acquisition or sort is embedded in the FCS file. The file can be opened
at a later time for analysis or to be used as a template for a subsequent acquisition.
4.5.7 Saving Partial FCS Files
There may be instances when a large number of events will be acquired during a sort. These
FCS files can become very large and potentially can cause the software to run more slowly
when reopening for analysis. In addition, it may be difficult to view populations with large
numbers of events displayed at one time. It is possible to save a partial FCS file during a sort by
saving only a set number of events (Figure 47). Click
File > Save Partial FCS File . A window
will appear to select how many events to save. The selected number of events will be saved
from the end of the sort. Sort statistics from the entire sort will be saved regardless of the partial
of the FCS data saved.
For example, if 500,000 events are sorted and 20,000 events are saved as a Partial FCS file,
then the last 20,000 events sorted (480,001–500,000) will be saved to the file.
Fig. 47. Save Partial FCS File Events window.