BIO RAD 145-1001, Instruction Manual

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6.2 Sort Modes
Table 24 describes the three sort modes available when setting up a sort. Sort modes can be
selected for each sort direction.
Table 24. Sort mode definitions.
Sort Mode Definition When to Use
Single Cell This mode requires that one and only one positive event be in the Single cell deposition
center of the droplet without any negative events nearby. Recovery
will be reduced due to the sort restrictions
Purity This mode will sort as long as no negative events are present in the General sorts where both purity
droplet. If multiple positive events are present, sorting will still occur and recovery are important
Enrich This mode will sort all positive events regardless if negative events Sorts when recovery is critical
are nearby. Purity will be reduced to maintain high recovery and purity is not as important
6.3 Sort Statistics
The sort statistics window can be viewed during
a sort and added to a print page for reporting
(Figure 75). This window will automatically open
when a sort is started.
If a saved partial FCS file is opened, aggregate
statistics can be shown:
1.
File > Open FCS File with Protocol .
2. Click on the Home tab and click Sort
Statistics .
3. Check the Show Aggregate Statistics box
to view statistics from all sorted events.
Fig. 74. Sort Logic window with sort priority and sort modes highlighted.
Fig. 75. Sort Statistics window.