BIO RAD 145-1001, Instruction Manual

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| 11 S3 Cell Sorter Instruction Manual | 11
2.4 Electronics
The S3 electronics process and deliver the emitted light signals to the software for user
analysis. Also included in this subsystem are the deflection plates in the sort chamber and the
safety interlocks.
CAUTION! Shock hazard! Only qualified, trained technicians should carry out
service work on electronic components due to potential shock hazard.
2.4.1 Interlocks
To prevent exposure to laser light and shock hazards, the S3 system is equipped with safety
interlocks (Figure 10). On the instrument control panel of the software, the instrument status
box will show visual indicators if these interlocks have been disengaged.
Note: When opened, the nozzle door will close the laser shutters to prevent laser light
exposure. This interlock will also turn off the drop drive and the stream.
IMPORTANT!
Open this door only if required to clear a clog or change the nozzle tip.
The QC procedure must be repeated to readjust the alignment and reset the drop
delay if this door is opened.
The sort door interlock will disable the plate voltage. In addition, if sample is running it will
be stopped.
2.4.2 Pre-Amplifier
The pre-amplifiers are used to boost the signal coming from the PMT.
2.4.3 ADCs
Analog to digital converters (ADCs) convert the electrical signal coming from the pre-amplifier
into a digital signal and transfer that signal to the software for visualization of the data.
Fig. 10. S3 safety interlocks.
Laser shutter interlock
Sort door interlock