BIO RAD 145-1001, Instruction Manual

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| 41 S3 Cell Sorter Instruction Manual | 41
4.4.6 Recalculate Drop Delay
To recalculate the drop delay using ProLine calibration beads, click the
Recalculate
button
(Figures 40 and 41). This button will not align the streams or verify CVs. This button will only
calculate the drop delay.
During the drop delay calculation in the QC procedure, the same recalculate button on the
Drop Delay Alignment Status window will be available in the Drop Monitor window. During the
QC procedure, recalculation can be performed until values are acceptable, prior to clicking the
checkmark box.
4.4.7 QC Criteria
Data collected during the QC procedure are checked against the QC criteria after the system
has been aligned. Users with administrator privileges may access and modify these settings in
the Administrator tab. For more information, refer to Section 3.3, Administrator Tab Toolbar.
CAUTION! Adjusting any of these values will affect the overall system performance.
For each fluorescent parameter, two criteria may be set:
n
PMT voltage — the PMT voltage required to bring the population to channel 128 must be
below the set point for each parameter
n
Coefficient of variation (CV) — the CV is measured in each parameter and must be under
the set point
IMPORTANT! These values may need to be changed to account for lot-to-lot
variation of the ProLine calibration beads.
4.4.8 QC Report
A QC Report window will open after
completing the alignment and drop
delay settings. This window displays the
QC status and whether the system has
passed or failed the QC requirements.
This report can be saved or printed
(Figure 42).
To view a previous QC Report, on the
Setup and Maintenance tab select
QC
Report > Date .
Fig. 42. QC Report window with QC status highlighted.
Print report
Open FCS file
Save data file